In-Vitro Screening of Phyllanthus Amarus and Eclipta Alba Against Leptospira Autumnalis

Title: In-Vitro Screening of Phyllanthus Amarus and Eclipta Alba Against Leptospira Autumnalis

Authors: Arthi Mohan, Saradhai Pandurangan, Saravanan Ramalingam

DOI: http://dx.doi.org/10.18535/jmscr/v4i5.38

Abstract

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Abstract

Background and Study Aims: Multi-drug resistance is one of the major public health problems especially in developing countries where relatively easy availability and higher consumption of medicines have lead to disproportionately higher incidence of inappropriate use of antibiotics and greater levels of resistance compared to developed countries. Leptospirosis is the most wide spread zoonoses in the world and recognized as an important public health problem.

Aim and Methods: To extract and identify some phytochemicals from the commonly available and widely studied Indian medicinal plants (Phyllanthus amarus and Eclipta alba) with anti-bacterial, hepatoprotective and renoprotective properties and also to conduct an anti-leptospiral susceptibility study on MIC of a most common Leptospira interrogans serovar autumnalis following the standard TDT.

Results: The present study revealed the presence of tannins, alkaloids, saponins, flavonoids, terpenes and anthraquinones in the leaves of these plants extracts thereby exhibiting anti-leptospiral activity. The drug dose concentration the extracts of P. amarus was found to be having a better active principle (160 μg/ml) than the extracts of E. alba (320 μg/ml).

Keywords: Leptospira autumnalis, P. amarus, E. alba, Tube dilution technique.

Abbrevations: TDT= Tube Dilution Technique, MIC= Minimum Inhibitory Concentration.

References

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