Induced To Differentiation and Morphogenesis of Pancreatic Islet Clusters: Effects on Glycated Fetal Bovine Serum

Title: Induced To Differentiation and Morphogenesis of Pancreatic Islet Clusters: Effects on Glycated Fetal Bovine Serum

Authors: Sivalingam Murugan, Ikuo Nishigaki, Gowri Rangasamy Gunassekaran, Ganapathy Ekambaram, Kalpana Deepa Priya Dorayappan, Vishal Babu, Ameesh Madhusoodhanan Nair

DOI: http://dx.doi.org/10.18535/jmscr/v4i5.17

Abstract

References

Corresponding Author

Abstract

Mouse embryonic stem cells (MESCs) are potency to differentiate into mature pancreatic islet cells and using for tissue repair treatment. Advanced glycation end-products (AGEs) are accrual of long lived proteins of various tissues and have been implicated in chronic complications in diabetes mellitus, but the pathogenic role of AGEs on pancreatic islet cells is remains unclear. Stem cell differentiation is carried out based on standard protocol followed in the laboratory. In results the differentiated stem cells shows extensive self-renewal capacity and grow in highly viable for long term in the culture media, had remarkable size, formed aggregates, which built tissue like structures and closer examination by light microscopy analysis revealed islet cell like morphology. Administration of GFBS +iron on induced pancreatic islet cells is observed induce charity of cells. Conclusion this ﬁndings demonstrate that AGEs directly cause insulin secretory defects and toxicity, most likely by impairing pancreatic cell functions, which may contribute to the development of diabetes. This approach offers a promising an ample supply of insulin producing cells for cell transplantation therapy and maintains the complication factors in diabetic mellitus, suggesting that further improvement is needed.

Keywords: Embryonic stem cell, insulin producing cell, diabetes, Glycated bovine albumin

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Call For Paper Volume 12 Issue 12 December 2024

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