Title: A Comparative study on staining for Helicobacter Pylori using new triple stain with routine Hematoxylin-Eosin and Giemsa stain
Authors: Krishna Sruthi R P, Preethamol S, Indira K
DOI: https://dx.doi.org/10.18535/jmscr/v8i2.110
Abstract
Background: Helicobacter pylori, a spiral-shaped bacterium, can be seen in hematoxylin and eosin staining. However, when a low density of Helicobacter pylori and atrophic mucosal change are combined, it becomes difficult to demonstrate the organism. Hence many Pathologists now use Hematoxylin-Eosin plus a second stain for the bacteria. The aim of this study is to develop a New Triple Stain (Carbol Fuchsin/ Alcian Blue/ Hematoxylin-Eosin) is suitable for simultaneous visualization of Helicobacter pylori and gastric morphology.
Objective: To introduce a New triple stain for simultaneous visualization of gastric morphology and Helicobacter pylori and compare it with routine Hematoxylin-Eosin and Giemsa.
Method: The study was carried out in Histopathology Laboratory and Dept. of MLT in Govt. Medical college Thiruvananthapuram. A total of 47 gastric biopsies confirmed as positive and negative by PCR were included in this study. Each sections from these biopsies stained with Hematoxylin-Eosin, Giemsa and New Triple stain and evaluated by the pathologist.
Result & Conclusion: The study was aimed to compare Hematoxylin-Eosin, Giemsa and New Triple for the detection of H. pylori. Morphological details are well observed in Hematoxylin-Eosin stain when compared to New Triple stain but bacterial demonstration is poor. Giemsa stain shows poor morphology when compared to both Hematoxylin-Eosin and New Triple Stain but bacterial identification is superior to Hematoxylin-Eosin. Hence it can only be used as a special stain for H.pylori. In histopathological aspect New Triple Stain shows greater sensitivity followed by Giemsa & Hematoxylin-Eosin.
Keywords: PCR, Hematoxylin-Eosin, Giemsa, New Triple Stain.
