Abstract
Introduction: Mosquito-borne diseases are the major concern in public health problems. Malaria is a protozoal disease caused by the parasites of the Genus Plasmodium - which are transmitted by the bite of female Anopheles mosquitoes. Prevalence of malaria worldwide is about 35%. Malaria infection diagnosed by Peripheral smear, Rapid Immunochromatography, and Molecular technique.
Aim and Objective: To study the epidemiological distribution of Plasmodium infection in patients suspected for Pyrexia of Unknown Origin (PUO) attending a tertiary care hospital in Kanchipuram District, Tamilnadu.
Methodology
Study Design: This Study was carried out at SRM MCH & RC, Tamil Nadu, India, from January 2018 to January 2019. This was an observational study.
Malarial parasites were identified in a peripheral blood smear by the conventional method (thick smear and thin smear), Rapid Immunochromatographic technique (a serological technique using whole blood) and Molecular technique. DNA extraction from whole blood done by Spine Column technique. Amplification and Gel Documentation done.
Result: Total of 83 blood samples were collected from patients with a clinical diagnosis of PUO. Out of 83 samples, 5(6%) samples were positive for Plasmodium vivax species by conventional method, Rapid Immunochromatographic technique. The Molecular technique will help to diagnose the gene.
Conclusion: In this study, Plasmodium vivax is identified more by conventional peripheral blood smear method as well as in Rapid Immunochromatographic method and Molecular Method.
Keywords: Epidemiology, Malaria, Plasmodium, Mosquito-borne, Immunochromatography.
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Corresponding Author
Sourav Das
SRM Medical College Hospital & Research Centre, Kattankulathur, Chennai, Tamilnadu, India
DOI: https://dx.doi.org/10.18535/jmscr/v7i6.77
