Abstract
Introduction: Tuberculosisis caused by infection with members of Mycobacterium tuberculosis complex, which is rigorous and contagious disease. Bacterial infection is one of the most essential complications in the patients with pulmonary tuberculosis.
Aim and Objective: To study the prevalence of multidrug resistant Gram-negative bacteria causing respiratory infections in pulmonary tuberculosis.
Materials and Methods: This Study was carried out at SRM MCH & RC, Tamil Nadu, India, from January 2017 to February 2018 after the Institutional Ethical committee approval. This was a Cross sectional study. The samples are collected under aseptic conditions. Gram negative bacilli isolation done by conventional culture method and antibiotic sensitivity done by disk diffusion method (Kirby-Bauer disc diffusion method).
Result: Out of 73 samples 10 (14.4%) samples were culture positive for gram negative bacilli. The isolates obtained in positive culture are Klebsiella pneumoniae (5) 7.2%, Pseudomonas aeruginosa (3)4.3%, Acinetobacter baumannii (2) =2.8%.
Discussion: Pulmonary tuberculosis is one among the most common infectious diseases affecting humans worldwide. They are important causes of morbidity and mortality for all age groups, and each year approximately 7 million people die as a direct consequence of acute and chronic respiratory infections
Conclusion: In this study the prevalence of MDR-GNB is 14.4%.The predisposing factors of Mycobacterium tuberculosis patients are nutrition deficiency and immune compromised status. Klebsiella pneumoniae followed by Pseudomonas aeruginosa and Acinetobacter baumannii were the most common gram-negative bacteria isolated in cultures from lower respiratory tract in pulmonary tuberculosis patients.
Keywords: Multidrug resistance, Gram negative bacteria, Mycobacterium tuberculosis.
References
- Woodhead M, Blasi F, Ewig, S, Garau J, Huchon G, Leven M. Guidelines for the management of adult lower respiratory tract infections-Full version. Clin Microbiol Infect 2011;17 Suppl 6:E1-59.
- Aurora Epv, D’ Agata EMC. (2005) The Rising Influx of Multidrug-Resistant Gram-Negative Bacilli into a Tertiary Care Hospital. Clinical Infectious Diseases.
- Gandhi NR, Nunn p, Dheda K, Schaaf HS, Zignol M, van Soolingen D, et,al (2010) Multidrug-resistant and extensively drug-resistant tuberculosis: a threat to global control of tuberculosis. Lancet 375:1830-1843, doi.
- Koneman’s Color Atlas and Textbook of Diagnostic Microbiology seventh edition Gary W. procop Deirdre L.Church.et.al
- Robert WA, Catharine N, Ruth G, Sherwin KA. Endemic aminoglycoside resistance in gram-negative bacilli: epidemiology and mechanisms. J Infect Dis 1980; 141:338-45.
- Anshum Aneja Arora; Uma Maheswari Krishnaswamy; and Mantha Satya Padmaja; Tubercular and bacterial co infection: A case series
- Christopher Aye Egbe1, Casimir Ndiokwere1, Richard Omoregie1,2 Microbiology of Lower Respiratory Tract Infections in Benin City, Nigeria
- Revised National Tuberculosis Control Programme (RNTCP) manual for laboratory techniciansCentral TB Division, Directorate General of Health ServicesMinistry of Health and Family Welfare, Nirman Bhavan, New Delhi 110 011.
- Egbagbe EE, Mordi RM. Aetiology of lower respiratory tract infection in Benin City, Nigeria. J Med Biomed Res. 2006;5(2):22–27.
- MARK H. LEPPER, M.D.**Chicago, Illinois Opportunistic Gram-Negative Rod Pulmonary Infections
- Carroll KC. Laboratory Diagnosis of Lower Respiratory Tract Infections: Controversy and Conundrums. J Clin Microbiol 2002; 40:3115-3120
- Shah NS, Wright A, Bai GH, et al. Worldwide emergence of extensively drugresistant tuberculosis. Emerg Infect Dis 2007; 13:380-7.
- Okesola AO,Ige OM. Trends in bacterial pathogens of lower respiratory tract infections.Ind J Chest Dis All Sc 2008;50:269-272.
- Kim H-r, Hwang S.S; Kim E—c; Lee S.M; Yang S-cYoo C-G; et al.(2011) Risk actors for multidrug-resistance bacterial infection among patients with tuberculosis. Journal of Hospital Infection.
- Antibiotic sensitivity pattern of gram negative bacilli isolated from the lower respiratory tract of ventilated patients in the intensive care unit Nidhi Goel, Uma Chaudhary, Ritu Aggarwal, and Kiran Bala Indian J Crit Care Med. 2009 Jul-Sep; 13(3): 148–151.
Corresponding Author
Manonmoney Jayaraman
Department of Microbiology, SRM Medical College Hospital and Research Centre, Kattankulathur, Chennai, Tamilnadu, India
DOI: https://dx.doi.org/10.18535/jmscr/v7i5.144
