Abstract
Introduction
Intraoperative cytology is a common technique used in the diagnosis of CNS neoplasms. Similarly, Imprint cytology technique in breast lesions is a simple and cost effective on table diagnostic tool where facilities for the commonly used frozen section studies are not available.1-6 Imprint is comparable to frozen section as a tool for intra operative and post-mortem diagnosis7-10 In this study we evaluate the accuracy of imprint cytology in rapid on table diagnosis of breast lesions in comparison with histopathology.
Subjects and Methods
The present study is a cross sectional study performed on all specimens of breast lesions received over a period of 17 months from September 2012 to January 2014. All breast specimens including mastectomy, lumpectomy, excision are included in the study with the exclusion of trucut biopsy. A total number of 71 cases were obtained.
Clinical and gross details of the patients were recorded. Imprint cytology of freshly excised tissue is prepared and stained with toluidene blue and Giemsa stains. The smears were studied and specific cytological diagnosis were made independently. The imprint smear findings are categorized into benign and malignant groups. Routine hematoxylin and eosin staining were done on tissue sections after fixation and histopatho-logical diagnosis were made for the lesions. Taking histopathology as a gold standard, sensitivity, specificity, positive predictive value and negative predictive value for diagnosing malignant lesions by imprint cytology are calculated.
References
- Ammar CA et al. Intra- operative cytology and frozen sections of breast lesions: a comparison from a Saudi teaching hospital. Bahrain Medical Bulletin 1996; 18:1.
- Hiregoudar AD, Godhi AS, Malur PR, Gogeri BV, Metgud SC. Accuracy of intra-operative imprint smears in breast tumours: A study of 40 cases with review of literature. Indian Journal of Surgery 2006;68:302-305.
- Kazuhiro S et al. Usefulness of intraoperative touch smear cytology in breast- conserving surgery. Experimental and therapeutic medicine 2010; 1:641-45.
- Hassanain HK et al. Role of imprint cytology in breast lesions. Iraqi J Med Sci 2009; 7(4):61-66.
- Maria F et al. Imprint cytology on microcalcifications excised by Vacuum-assisted breast biopsy: a rapid preliminary diagnosis. World Journal of Surgical Oncology 2007, 5:40.
- Kashiwagi S et al. Adjunctive imprint cytology of core needle biopsy specimens improved diagnostic accuracy for breast cancer. SpringerPlus 2013; 2:372.
- Sahil IP et al. Utility of fine needle aspiration cytology in the evaluation of breast lesions. Journal of Clinical and Diagnostic Research. 2013; 7(12): 2777-2779.
- Sachin SK et al. Role of scrap cytology in the intraoperative diagnosis of tumor. J Cytol 2010; 27(3):86-90.
- Sushma NR et al. Imprint smear cytology and histopathology of breast lesions - a comparative evaluation with review of literature. Cibtech Journal of Bio-Protocols 2012; 1 (2):22-27.
- Akbar S et al. Comparing touch imprint cytology, frozen section analysis, and cytokeratin immunostaining for intraoperative evaluation of axillary sentinel lymphnodes in breast cancer. Indian J Pathol Microbiol 2012; 55(2).
- Khanna R, Khanna S, Chaturvedi S and Aryya NC. Spectrum of breast disease in young females – A retrospective study of 1315 patients. Indian J Pathl Microbiol 1988;41(4):379-401.
- Malik R, Bharadwaj VK. Breast lesions in young females-- a 20-year study for significance of early recognition. Indian J PatholMicrobiol 2003;46(4):559-562.
- El Saghir et al. Age distribution of breast cancer in Lebanon: increased percentages and age adjusted incidence rates of younger-aged groups at presentation. J Med Liban.2002;50(1-2):3-9.
- Stanley MW et al. Current issues in breast cytopathology. Am J Clin Pathol 2000;113 (1):S49-S75.
- Shah SH et al. Diagnostic evaluation of fine needle aspiration cytology in the management of palpable breast lesions. JPMA 1998;48:7.
- Nandhini NM et al. Evaluation of scoring system in cytological diagnosis and management of breast lesion with review of literature. Indian Journal of Cancer 2011; 48(2).
- Dudgeon LS, Barrett NR. The examination of fresh tissues by the wet film method. British Journal of Surgery 1934; 22:4-22.
- Prakash HM et al. The value of systemic pattern analysis in FNAC of breast lesions: 225 cases with cytohistological correlation. J Cytol 2011; 28(1):13-19.
- Tribe CR. Cytological diagnosis of breast tumors by the imprint method. Journal of Clinical Pathology 1965;18:31-39.
- Haeri H et al. Comparision of the cytology technique and the frozen section results in intraoperative consultation of the breast lesions. Acta Medica Iranica 2002; 40(3):203-206.
- Akthar ZM et al. Evaluation of accuracy of intra operative imprint cytology for detection of breast lesions. Annals 2010; 16(1).
- Lee TK. The value of imprint cytology in tumor diagnosis. A retrospective study of 522 cases in northern China. ActaCytologica 1982;26:169-171.
- Dutta SK, Chattopadhyaya A, Roy S. Evaluation of fine needle aspiration and imprint cytology in early diagnosis of breast lesions with histopathological correlation. Journal of Indian medical Association 2001;99(8):421-3.
- Scopa CD et al. Tissue imprints in surgical pathology: a rapid intraoperative diagnostic aid. DiagnCytopathol 1990; 6(1) :5-8.
- Suen KC, Woods WS, Syed AA, Quenville NF, Clement PB. Role of imprint cytology in intra operative diagnosis: value and limitations. Journal of Clinical Pathology 1978;31:328-337.
- Mortensen E, Hansen L. Imprint as a reliable diagnostic tool in breast cancer and possible usefulness for research purposes. Breast cancer research 2005; 7(2):3.
- Anastasiadis P, Romanidis K. Proliferative breast disease: epidemiological aspects and cytological diagnosis. European J GynaecolOncol 2003; 24(6):547-51.
- Gray W, McKee GT. Diagnostic cytopathology 8th 2004;p 648-9.
- Tarek MN et al. Intra-Operative Diagnosis of Breast Mass-Lesions: Comparison of the validity of touch smear preparation and frozen section techniques. Journal of the Egyptian Nat. Cancer Inst. 2008; 20(1):63-69.
- Rakesh M, Ashok P et al. Study of imprint smears of various lesions with histological correlation. International journal of medical science and public health 2014; 3(4).
Corresponding Author
Dr Ronald J. Bosco
Trichy SRM Medical College
Email: This email address is being protected from spambots. You need JavaScript enabled to view it., Mobile: 9489214244
DOI: https://dx.doi.org/10.18535/jmscr/v7i1.192
