Abstract
Aim: The aim of this study was to evaluate the susceptibility profile of Candida species isolated from clinically relevant specimens by micro broth dilution method to fluconazole.
Setting of the study: The study was conducted in Department of Microbiology, Govt. Medical College, Kozhikode, Kerala over a period of one year.
Materials and Methods: Antifungal susceptibility of 117 relevant Candida isolates were done by microbrothdilution methods according to CLSI guide lines
Results: 96.6% of all Candida isolates were sensitive to fluconazole and 3.4% were found to be resistant.
Keywords: Candida albicans, Non albicans Candida, Fluconazole.
References
- Edward JE.Candida species :Mandell GL, Bennet JE, Dolin R, Mandell, Douglas and Bennetts, Principles and Practice of infectious diseases.&7th Ed. Churchil Livingstone;2010 p.3225-40
- M. Achkar and Bettina C. Fries, Departments of Medicine and Microbiology and Immunology,Albert Einstein College of Medicine, Bronx, New York, Clinical Microbiology Reviews, Candida infections of genitor urinary tract; American Society for Microbiolgy. Apr. 2010,Vol.23, p.253-73.
- Mohandas M. Ballal. M. Distribution of Candida Species in Different Clinical Samples and Their Virulence, Biofilm Formaton, proteinase and Phospholipase Production. A Study on Hospitalized Patients in Southern India. J. Glob. Infect.Dis.2011;3 (1) 4-8.
- Forbes BA, Sahm DF, Weissfeld AS, Laboratory methods in basic mycology In: Wilson L, Wurm E, editors, Bailey & Scott's Diagnostic Microbiology. 12th Ed.Mosby Elsevier,2007. p.700-13.
- Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenberger, P.C. 1997.Mycology. In: Color Atlas and Textbook of Diagnostic Microbiology, 5th edn. Lippincott Williams & Wilkins, Philadelphia,PA. p. 983-1057.
- Reference method for Broth Dilution Antifungal susceptibility Testing of Yeast; Approved Standard - CLSI M27-A3 Document 2008,Third edition,vol. 28 No.14 Clinical and Laboratory Standards Institute, Wayne.
- Varkadas, K Z,G. Samonis, A. Michalo-poulos, E.S. Soteriades and M.E. Falagas. 2006. Antifungal prophylaxis with azoles in high risk, surgical intensive care unit patients, Ameta analysis of randomized placebo controlled trials.Crit.Care.Med.34:1216-24.
- Jose Garnacho-Montero, Ana Diaz martin, Emiloio Garcia carbera, Critical care and emergency department, intensive care unit, Virgen del Rocio. Univesity hospital risk factors for fluconazole resistant Candidemia: Antimicrobial Agents And Chemo-therapy,Aug. 2010,Vol.54,No.8 3149-54
- Fothergill AW, Sutton DA, McCarthy DI, Wiederhold NP. Impact of new antifungal breakpoints on antifungal resistance in Candida J Clin Microbiol. 2014; 52: 994–997. doi: 10.1128/ JCM.03044-13. [PMC free article] [PubMed] [Cross Ref].
- Zhang L, Yang HF, Liu YY, Xu XH, Ye Y, Li JB. Reduced susceptibility of Candida albicans clinical isolates to azoles and detection of mutations in the ERG11 Diagn Microbiol Infect Dis. 2013;77:327–329. doi: 10.1016/j.diagmicrobio.2013.08.018. [PubMed][Cross Ref].
- Lockhart SR, Iqbal N, Cleveland AA, Farley MM, Harrison LH, Bolden CB, et al. Species identification and antifungal susceptibility testing of Candida blood-stream isolates from population-based surveillance studies in two US cities from 2008 to 2011. J ClinMicrobiol. 2012;50: 3435–3442. doi: 10.1128/JCM.01283-12. [PMC free article] [PubMed] [Cross Ref.
- Pfaller MA, Diekema DJ, Gibbs DL, Newell VA, Ellis D, Tullio V, et al. Results from the ARTEMIS DISK global antifungal surveillance study, 1997 to 2007: a 10.5 year analysis of susceptibilities of Candida species to fluconazole and voriconazole as determined by CLSI standardized disk diffusion. J ClinMicrobiol. 2010;48:1366–1377. doi: 10.1128/JCM.02117-09. [PMC free article] [PubMed][Cross Ref.
- Saharali Mohamed and ZiabZakey Al Ahmadey. Biofilm Formation and Anti-fungal susceptibility of Candida Isolatesfrom Various Clinical Specimens, British Microbiology Research Journal. 2013;3 (4):590-601.
- Adhikary. R, Joshi.S. Species distribution and antifungal susceptibility of Candidemia at a Multi superspeciality centre in South India. Indian J med microbial 2011;29:309-11
Corresponding Author
Dr Sameera.P
Assistant Surgeon, Govt. Taluk Hospital Kuttippuram, Kerala, India
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