Title: Qualitative and Quantitative Screening of total Phenolic and total Flavonoid for Different Extracts of Tridax procumbens L. and Comparative Study of their Anti-Oxidant Properties
Author: Dr Deepshikha Roy
DOI: https://dx.doi.org/10.18535/jmscr/v6i9.59
Abstract
The different solvent extracts such as Acetone extract, Ethyl acetate extract, 70% Alcoholic extract and Aqueous extract were first qualitatively screened for Flavonoids and Phenolics. Flavonoids and Phenol were present in every solvent extract of Tridax procumbens L. except that phenol was absent in acetonic extract. Highest concentration of Flavonoids and Phenolic were present in 70% alcoholic extract and ethyl acetate extract respectively with 398.33 mM equivalent of Quercetin and 343.66 mM equivalent of Gallic acid respectively. The acetonic extract had least quantity of Flavonoids and Phenolics with 38.33 mM equivalent of Quercetin and 160.33 mM equivalent of Gallic acid respectively. However this (acetonic) extract show comparatively higher antioxidant activity with 34.19% of radical scavenging activity followed by 70% alcoholic extract with 9.03% radical scavenging activity. Ethyl acetate and aqueous extract didn’t have any measurable radical scavenging activity.
Keywords: Flavonoids, Phenolics, DPPH assay.
