Pseudomonas aeruginosa is one of the most common opportunistic nosocomial pathogens, which causes a wide spectrum of infections and leads to increased mortality and morbidity. Due to indiscriminate use of antibiotics, significant changes in microbial genetic ecology is seen and this has led to the spread of multidrug resistance globally. The present study was undertaken to detect the extended spectrum β lactamases (ESBL) in Pseudomonas aeruginosa isolated from pus samples and to evaluate their susceptibility patterns. A total of 90 isolates of P.aeruginosa were analyzed to study their sensitivity patterns. The presence of the ESBL enzyme was detected by the phenotypictest-Double Disc synergy test. Out of 90 isolates of P.aeruginosa, 20(73.3%) were ESBL producers (of the 30 P.aeruginosa resistant to cetazidime). All the ESBL producing isolates were sensitive to Imipenemwhich is a carbapenem and is the drug of choice for treating infections caused by ESBL producing P.aeruginosa. Thus, we recommend a routine surveillance on antibiotic resistance in all hospitals so that control measures can be taken to prevent the spread of these strains in the hospitals at a very early stage itself.

Keywords: Pseudomonas aeruginosa, ESBL, Imipenem, DDST.

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Dr Soumya.S

Assistant Professor, Department of Microbiology,

Jawaharlal Nehru Medical College, Belgaum

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