Title: Detection of Extended Spectrum Beta-Lactamases in Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital
Authors: P R Lyra, Sindhu Cugati, K Anuradha, D Venkatesha
DOI: https://dx.doi.org/10.18535/jmscr/v5i4.94
Abstract
Background: Extended spectrum ß-lactamases (ESBLs) represent a major group of lactamases responsible for resistance, mostly produced by gram-negative bacteria, to newer generations of ß-lactam drugs currently being identified in large numbers worldwide. The aim of the present study was to detect the extended-spectrum β-lactamase (ESBL) production among Pseudomonas aeruginosa isolates from various samples.
Materials and Methods: A total of 100 Pseudomonas aeruginosa isolates from various samples in a tertiary care hospital from January 2011 to December 2011 were included in the study. Kirby-Bauer’s disc diffusion method was employed to detect the antibiotic susceptibility pattern of the isolates. The isolates were tested for ESBL production using double-disc synergy test (DDST) using ceftazidime alone and combined ceftazidime- clavulanic acid discs.
Result: Out of the 100 Pseudomonas aeruginosa isolates studied, 45 (45%) were ESBL producers .Out of the ESBL producing isolates, 44 (98%) were resistant to third generation cephalosporins, 28 (62%) to Ciprofloxacin ,34(76 %) to Gentamicin, 28(62%) to Amikacin respectively. 45(100%) of isolates were susceptible to Imipenem.
Conclusion: This study emphasizes on the need for global control of antimicrobial resistance; and to create awareness among the clinicians and general population thereby reducing the mortality and morbidity associated with multi-drug resistant pathogens.
Keywords: Pseudomonas aeruginosa, extended spectrum β-lactamases, third-generation cephalosporins, Kirby-Bauer disc diffusion technique, double-disc synergy test, Multidrug Resistance.
