Extended Spectrum Beta-Lactamases (ESBLs) are beta-lactamases resist against bacteria to Pencillins, Ceprhalosporins and Aztrenam (not to Cephamycins or Carbapenems) and inhibited by Beta-lactamase inhibitors such as Clavulanic Acid. ESBLs- producing strains can resist to Cephamycins due to loss of porin protein on the outer membrane. ESBLs enzyme coded by gene TEM1, TEM2 and SHV1 and due to presence of same genes in Pseudomonas aeruginosa, consider as wide spread reservoir of ESBL enzymes. Identication of P.aeruginosa based on colony morphology and it’s biochemical reactions from various positive clinical samples. Also, Double Disc Synergy Test has been performed for the detection of ESBL detection. Polymycin, Colistin have maximum sensitivity against P.aeruginosa. Among all positive samples, only 7% ESBL produced.ET secretion have high degree of isolation followed by urine and pus samples. Due to presence of Clavulanic Acid which act as Beta-lactamase inhibitor help to screen the production of ESBL, that’s why, only 7 % production of ESBLs production by Phenotypic Confirmatory Test combined Disk Diffusion Test. The present study help to prevent the ESBLs production by applying Clavulanic Acid as main drug of choice for physicians. 

Keywords: ESBL, Clavulanic Acid, Double Disc Synergy Test.