Title: Isolation, Characterization and Identification of L-Glutaminase, an Anticancer agent Producing Bacteria Occurring in Soil
Authors: Dr Praveen Reddy P, Ravi A, Gangadhar N, Inna Reddy. G
DOI: https://dx.doi.org/10.18535/jmscr/v7i7.13
Abstract
Microbes are the sources of various enzymes which are commercially important. Some enzymes, particularly L-glutaminase and L-asparaginase are medically important as they can be used for the treatment of leukemia. Many bacteria reported to produce L-glutaminase which can be used as an anticancer agent. L-glutaminase is used in the preparation of baked foods to increase the flavor of the foods. In the present paper five L-glutaminase producing bacteria were isolated from garden, market area and agricultural soils using minimal L-glutamine medium supplemented with phenol red indicator. Based on microscopic examination and biochemical characterization the bacteria were identified as Pseudomonas, Serratia, Proteus, Staphylococus and Bacillus species. These bacteria can be improved and exploited for the commercial production of L-glutaminase.
Keywords: L-glutaminase, leukemia, anticancer, L-glutaminase producing bacteria.
References
- Aravinth VJS, Moklesur RSM, Long CM, Marylin JPS, Ravikumar M, Wong TW. Enhancement of the production of L-glutaminase, an anticancer enzyme, from Aeromonas veronii by adaptive and induced mutation techniques. PLOS ONE. 2017; 12(8): 1-17.
- Tanti Y, Ekowati C, Usman SFT. Screening and Characterization of L-glutaminase produced by bacteria isolated from Sangihe Talaud Sea. Squalen. 2012; 7(3): 115-122.
- Savitha SD, Sonal JC, Basavaraj SH. Production, purification and characterization of L-glutaminase from Streptomyces Isolated from soil. Journal of Applied Pharmaceutical Science. 2016; 6(7): 100-105.
- Rajendra D, Anubrata P, Neha P. Isolation, Production & Screening of Anti-Cancer enzyme L-glutaminase from Bacillus subtilis. International Journal of Pharmacy and Biological Sciences. 2015; 5(2): 96-105.
- Sridevi S, Prathyusha K, Jagan MYSYV, Sandeep BV. Isolation and primary screening of L-glutaminase from agro-residual wastes. International Journal of Pharmaceutical Sciences Review and Research. 2016; 39(2) 147-150.
- Swathi S, Vinod KN. Production and characterization of L-glutaminase by Bacillus International Journal of Pharmaceutical Sciences and Research. 2016; 7(4): 1620-1626.
- Gulbahar FK, Karkaz MT. Determination of L-Glutaminase activity by some bacterial species. International Journal of Current Microbiology and Applied Sciences. 2016; 5(4):218-225.
- Suresh KS, Muthuvelayudham R, Viruthagiri T. Statistical Optimization based Production of L-Glutaminase (EC.3.5.1.2) by Serratia marcescens under submerged Fermentation. Research Journal of Chemical Sciences. 2013; 3(6): 43-53.
- Wade HE, Robinson HK, Phillips BW. Asparaginase and Glutaminase Activities of Bacteria. Journal of General Microbiology. 1971; 69: 299-312.
- Suresh T, Prakasham RS. Isolation and Identification of L-Glutaminase an Antilekeumic enzyme Producing Microorganism From Godavari River Bank Soils in Andhra Pradesh. International Research Journal of Pharmacy. 2010; 1(1): 367-373.