Protective Role of Vitamin C on Sperm Morphology and Biochemical Enzymes in Lead Nitrate Treated Albino Rats

Title: Protective Role of Vitamin C on Sperm Morphology and Biochemical Enzymes in Lead Nitrate Treated Albino Rats

Authors: Urvashi Singh, Priyanka Singhal, Archana Rani, Navneet Kumar, AK Srivastava

DOI: http://dx.doi.org/10.18535/jmscr/v4i8.89

Abstract

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Corresponding Author

Abstract

Lead is a well known heavy metal and is found in various components of earth environment and is a major industrial byproduct. Manipulation of lead from these sources causes toxicity in human beings by oxidative stress, which results in decrease fertility due to increase in the production of abnormal sperm population. Vitamin C is an antioxidant and acts by scavenging the free radicals, thus ameliorating the oxidative stress. The present study was carried out to evaluate the effect of lead nitrate and Vitamin C on testis. The study was conducted on 30 male albino rats of Wistar strain, weighing 150-250 gm. The rats were divided into 5 groups (6 rats in each group). Group 1 served as control and rest 4 groups were experimental. Except the control group, the animals of other 4 groups received lead nitrate and vitamin C orally for 6 days a week for 2 months. All the animals were sacrificed at the end of experiment and the result showed abnormal sperm count 5% in control group while it was 66% in group 4 and 17% in group 5. Sperm abnormalities observed were double head, banana head, amorphous head, defective head, headless, bent neck, bent tail, double tail, defective tail and looped tail. Malondialdehyde, a marker of lipid peroxidation was increased in experimental group while glutathione reductase (GR) enzyme was decreased in testicular tissue but this change in enzyme showed some improvement in vitamin C treated groups.

Key words: Lead nitrate, biochemical enzymes, sperm morphology, vitamin C.

References

1. Winder C. Reproductive and chromosomal effect of occupational exposure to lead on the male. Reprod. Toxicol., 1989; 3: 221-233.

2. Sokol RZ. Hormonal effects of lead acetate in the male rat. Mechanism of action. Biol. Reprod., 1987; 37: 1135-1138.

3. Thomas JA, Brogan WC. Some actions of lead on the male reproductive system. Am J Indus, 1983; 4(4): 127-34.

4. Rodamilans M, Osaba MJ, Figneras J, Rivera F, Margner JM, Peraz P, et al. Lead toxicity on endocrine testicular function in an occupationally exposed population. Hum Toxicol., 1988; 7(2): 125-8.

5. Saxena DK, Srivastara RS, Lal B and Chandra SV. The effects of lead exposure on the testis of growing rats. Exp. Pathol., 1987; 31: 240-52.

6. Flora SJS. Nutritional Components Modify Metal Absorption, Toxic Response and Chelation therapy. J. Nutr. Environ. Med., 2002; 12: 51-65.

7. Agarwal A and Allamaneni SS. Role of free radicals in female reproductive diseases and assisted reproduction. Reporod Biomed online, 2004; 9(3): 338-47.

8. Ding Y, Gonick HC, Vaziri ND. Lead promotes hydroxyl radical generation and lipid peroxidation in cultured aortic endothelial cells. Am. J. Hypertens., 2000; 13: 552-55.

9. Mudipalli A. Lead hepatotoxicity and potential health effects. Indian J Med Res., 2007; 126: 518-527.Abdel-Wahhab MA, Ahmed HH, Hagazi MM. Prevention ofaflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts. J. Appl. Toxicol., 2006; 26(3): 229-238.

10. Patra RC, Swarup D, Dwidedi SK. Antioxidant effects of á- tocopherol, ascorbic acid and L-methionine on lead-induced oxidative stress of the liver,kidney and brain in rats. Toxicol, 2001; 162: 81-8.

11. Flora SJ, Tandon SK. Preventive and therapeutic effects of thiamin, ascorbic acid and their combination in lead intoxication. Acta Pharmacol. Toxicol., 1986; 58: 374-378.

12. Goyer RA and Cherion MG. Ascorbic acid and EDTA treatment of lead toxicity in rats. Life Sci., 1979; 24: 433-438.

13. Anderson D and Francis AJ. The modulating effects of antioxidants in rat embryos and sertoli cells on culture. Basic Life Sci., 1993; 61: 189-200.

14. Grajeda-Cota P, Ramirez-Mares MV and De Mejia EG. Vitamin C protects against in vitro cytotoxicity of cypermethrin in rat hepatocytes. Toxicology in Vitro, 2004; 18: 13-19.

15. Ahmed YF., Karima GHMM, Farghaly AA, Mona A, Abo-Zeid and Ismail EM. Toxic effects of prolonged lead exposure in male rabbits: Chromosomal and Testicular alteration. Global Veterinaria, 2012; 8(4): 360-366.

16. Antnio, G., Joao, R.S., Maria de, and L.P. Effect of lead chloride on spermatogenesis and sperm parameters in mice. Asian J Androl, 2004; 6: 237-241.

17. Gautam AK, Agarwal K, Shah BA, Kumar S, Saiyed HN. Lead induced spermatoxicity in mouse and MPG treatment. J. Environ. Biol., 2001; 22: 287-91.

18. Lohiya NK, Manivannan B, Mishra PK, Pathak N, Sriram S, Bhande SS et al. Chloroform extract of Carica papaya seeds induces long-term reversible azoospermia in langur monkey. Asian J Androl., 2002; 4: 17-26.

19. Sarkar M, Chaudhuri GR, Chattopadhyay A, Biswas NM. Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J. Androl., 2003; 5: 27-31.

20. Naha N, Bhar RB, Mukherjee A and Chowdhury AR. Structural alteration of spermatozoa in the persons employed in lead acid battery factory. Indian J Physiol Pharmacol, 2005; 49(2): 153–162.

21. Wadi SA, Ahmad G. Effects of lead on the male reproductive system in mice. Journal of Toxicology and Environmental Health, 1999; 56(7): 513-521.

22. Acharya UR, Acharya S. and Mishra M. Lead acetate induced cytotoxicity in male germinal cells of Swiss Mice. Industrial Health, 2003; 41: 291-294.

23. Tohamy El MM and Nattat El WS. Effect of antioxidant on lead induced oxidative damage and reproductive dysfunction in male rabbit. Journal of American Science, 2010; 6(11).

24. Shabani A, Rabbani A. Lead nitrate induced apoptosis in alveolar macrophages from rat lung. Toxicology, 2000; 149(2-3): 109-114.

25. Abdel-Wahhab MA, Aly SE. Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis. J. Appl. Toxicol., 2005; 25(3): 218-223.

26. Abdel-Wahhab MA, Ahmed HH, Hagazi MM. Prevention ofaflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts. J. Appl. Toxicol., 2006; 26(3): 229-238.

27. Abdel-Wahhab MA, Omara EA, Abdel-Galil MM, Hassan NS, Nada SA, Saeed A, Elsayed MM. Zizyphus spina-christi extract protects against aflatoxin b(1)-intitiated hepatic carcinogenicity. Afr. J. Tradit.Complement Altern. Med., 2007; 4(3): 248-256.

28. Moneim AE, Dkhil MA and Al-Quraishy S. The potential role of flaxseed oil on lead acetate induced kidney injure in adult male albino rats. African Journal of Biotechnology, 2011; 10(8): 1436-1451.

29. Turguta G, Enlib Y, Kaptanoglub B, Turguta S, Genca O. Changes in the levels of MDA and GSH in mice serum, liver and spleen after aluminum administration. East. J. Med., 2006; 11: 7–12.

30. Beytut E, Yuce A, Kamiloglu NN, Aksakal M. Role of dietary vitamin E in cadmium-induced oxidative damage in rabbit’s blood, liver and kidneys. Int. J. Vitam. Nutr. Res., 2003; 73, 351–355.

31. Ognjanovic BI, Pavlovic SZ, Maletic SD, Zikic RV, Stajn AS, Radojicic RM, Saicic ZS, Petrovic VM. Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium. Physiol. Res., 2003; 52: 563–570.

32. Asayana K, Hayashibe H, Dobashi K, Niitsu T, Miyao A, Kato K. Antioxidant enzyme status and lipid peroxidation in various tissue of diabetes and starved rats. Diabetes Res., 1989; 12: 85-91.

33. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals induced renal and testicular injuries in male mice. Saudi Journal of Biological Sciences, 2011;18: 63-72.

34. Murray RK. Metabolism of Xenobiotics. In: “Harper’s Biochemistry “Murray RK, Mayes PA, Granner DK and Rodwell VW (eds), Appleton and Lange, Middle East Edition, California, 1991.

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