Protective Role of Vitamin C on Sperm Morphology and Biochemical Enzymes in Lead Nitrate Treated Albino Rats

Title: Protective Role of Vitamin C on Sperm Morphology and Biochemical Enzymes in Lead Nitrate Treated Albino Rats

Authors: Urvashi Singh, Priyanka Singhal, Archana Rani, Navneet Kumar, AK Srivastava

DOI: http://dx.doi.org/10.18535/jmscr/v4i8.89

Abstract

References

Corresponding Author

Abstract

Lead is a well known heavy metal and is found in various components of earth environment and is a major industrial byproduct. Manipulation of lead from these sources causes toxicity in human beings by oxidative stress, which results in decrease fertility due to increase in the production of abnormal sperm population. Vitamin C is an antioxidant and acts by scavenging the free radicals, thus ameliorating the oxidative stress. The present study was carried out to evaluate the effect of lead nitrate and Vitamin C on testis. The study was conducted on 30 male albino rats of Wistar strain, weighing 150-250 gm. The rats were divided into 5 groups (6 rats in each group). Group 1 served as control and rest 4 groups were experimental. Except the control group, the animals of other 4 groups received lead nitrate and vitamin C orally for 6 days a week for 2 months. All the animals were sacrificed at the end of experiment and the result showed abnormal sperm count 5% in control group while it was 66% in group 4 and 17% in group 5. Sperm abnormalities observed were double head, banana head, amorphous head, defective head, headless, bent neck, bent tail, double tail, defective tail and looped tail. Malondialdehyde, a marker of lipid peroxidation was increased in experimental group while glutathione reductase (GR) enzyme was decreased in testicular tissue but this change in enzyme showed some improvement in vitamin C treated groups.

Key words: Lead nitrate, biochemical enzymes, sperm morphology, vitamin C.

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