Title: Evaluation of the Influence of Storage time in the Determination of Serum Adenosine Deaminase Activity
Authors: Dr Radhika Krishnaswamy, Sr Daisy Kunnathupurambil, Priyadharshini K
DOI: http://dx.doi.org/10.18535/jmscr/v4i9.36
Measurement of adenosine deaminase (ADA) activity in various biological fluids is useful for the diagnosis of tuberculosis .It is a rapid and noninvasive test unlike the other routine tests used for the diagnosis of tuberculosis especially extra pulmonary tuberculosis. The time limit at which the analysis is done after the sample collection is crucial because the activity of the enzyme can decline with time at ambient temperature. The purpose of the present study is to identify, test, and determine the influence of storage time in the determination of serum adenosine deaminase activity and optimize the time at which the activity of ADA is best, consequently eliminating the error that can occur on delayed testing.. Serum samples collected from 20 healthy individuals both male and females in the age group of 20-60 years was used for the study Serum samples were analyzed on the same day of collection and then stored at -200C and the analysis was done on the following days 3,7,10,28. ADA analysis was done by the colorimetric procedure of Guisti and Galanti employing reagents optimized by Kaplan. All chemicals employed as stabilizing agents were of reagent grade. Results showed significant decline in the enzyme activity as the number of days on storage increases even though it is stored at -200C. The study shows that on storage without any additives there is a significant decline of the enzyme activity and it is advisable to do the analysis on the same day of sample collection. Keywords: ADA – Adenosine Deaminase, storage, day, enzyme, activity.
Abstract
