Abstract
Background: polyomaviruses are human virus associated with acute viral gastroenteritis globally and an essential cause of childhood morbidity and mortality in developing countries. However, there are still few published data on the happens of these viruses in Sudan. This study was conducted to investigate the molecular epidemiology of polyomaviruses in children less than 5 years with acute gastroenteritis.
Methods: A total of 208 stool samples were collected from hospitalized children under five years of age presenting with acute gastroenteritis, in Khartoum State, Sudan, during the period from November 2017 – May 2021, For the detection of Polyomavirus DNA from stool samples was extracted using Qiagen DNA kits from all samples, followed by Multiplex nested Polymerase Chain Reaction (PCR)
Results: The study revealed that 26 (12.5%) were positive for Polyomavirus. The study found no significant difference in the positivity of Polyomavirus according to the gender (p values= 0.66). Moreover, the study found no significant difference in the positivity of Polyomavirus according to the status of the polio-vaccination (p values= 0.946). The study showed that the majority of positive cases of Polyomavirus were younger than 4 years, with significant association (p < 0.001), The current study showed that there was strong association between Polyoma virus and gastroenteritis diseases (p. value = 0.00)
Conclusions: The present study showed that Polyoma virus are an important causative agents of gastroenteritis in children less than 5 years .There is a great need for introducing routine Polyoma virus testing of hospitalized children with gastroenteritis.
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Corresponding Author
Shaza Salih
DOI: https://dx.doi.org/10.18535/jmscr/v10i12.07
