Abstract
Isocyanates (ICN) have drawn considerable attention in the recent past as they can react with macromolecules (polypeptides, nucleic acids etc.) and produce toxic insults. ICN react with side chain nitrogen of amino acid residues of polypeptides. Pathophysiological implications resulting from occupational and accidental exposures of ICN are yet too elusive. It may be produced in high concentrations in conditions of chronic renal failure and chronic inflammatory diseases (diabetes mellitus, rheumatoid arthritis etc.). In this study, bovine serum albumin (BSA) has been carbamylated and characterized by UV and AGE. Antibodies against carbamylated-BSA were raised in animals and characterized by direct binding and inhibition ELISA. Presence of anti-carbamylated-BSA autoantibodies in sera of diabetic nephropathy patients was elucidated by ELISA. Carbamylated-BSA exhibited hyperchromicity at 280 nm compared to native BSA. Carbamylated-BSA was found to be highly immunogenic as compared to native BSA. Experimental induction of antibodies against carbamylated-BSA and presence of autoantibodies against carbamylated-BSA in diabetic nephropathic patients’ sera points towards the role for carbamylated-BSA in diabetic nephropathy.
Keywords: Isocyanates; Carbamylated-BSA; Autoantibodies; Diabetic nephropathy patients.
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Corresponding Author
Dr Sangita Choudhary, M.D.
Department of Biochemistry, Katihar Medical College, Al-Karim University, Katihar, Bihar, 854109, India
DOI: https://dx.doi.org/10.18535/jmscr/v10i10.16
