Abstract
Background: A biofilm is an accumulation of microorganisms embedded in an exopolysaccharide matrix of microbial host origin called polysaccharide intracellular adhesion (PIA). They pose a significant threat to indwelling devices, causing slow persistent infections, thereby interfering with antimicrobial therapy.
Objectives: This study was conducted to isolate and compare three conventional methods for the detection of Biofilm formation and their antimicrobial susceptibility pattern.
Materials and Methods: A total of 120 urinary catheter tips were collected from medical and dialysis units from May 2019 to November 2019. Biofilm production was detected by Congred agar, Tube method, Tissue culture methods. Antibiotic susceptibility test of Biofilm producing organisms was performed according to CLSI guidelines.
Results: Out of 120 samples 52 (43.33%), showed culture positivity, out of which bacteria are 37(71.2%) which includes E.coli 15(28%), klebsiella 10(19%), pseudomonas 6(11.5%), proteus 4(7%), Enterococci 2 (3.8%), and fungal culture revealed Candida 15 (28.8%). Out of 52 culture positives, 23(44.23%) produced Biofilm. The rate of detection by CRA, TM TCP methods was 23%, 36%, 44%, respectively. Higher antibiotic resistance was observed in Biofilm producing bacteria.
Conclusion: The TCP method of biofilm detection is quantitative and more reliable than TM and CRA methods; it can be recommended as a general screening method for the detection of Biofilm producing bacteria in laboratories.
Keywords: Biofilm of urinary catheter tips, Nosocomial infections, TCP, TM, CRA methods.
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Corresponding Author
Dr T. Parvathi
Department of Microbiology, Andhra Medical College, Visakhapatnam, India
DOI: https://dx.doi.org/10.18535/jmscr/v9i1.06
