Abstract

Objective: The aim of present study was to determine the spectrum of Non-fermenters in clinical sample and their antimicrobial Sensitivity pattern.

Material and Methods: A total of 3652 different types of clinical samples were received in our department from different OPD and IPD for culture and Sensitivity testing. Isolation, Identification and antibiogram of Isolates were performed using a standard protocols or CLSI guidelines.

Results: A total of 158 Non-fermenters Gram Negative Bacilli (NFGNB) was isolated from 3652 samples accounting an isolation rate of 4.32%. Psudomonas aeruginosa was the most common nonfermenters 98 (62.02%), followed by Acinetobactor baumannii 38(24.05%), Psudomonas fluorescens 16(10.12%) and Acinetobactor Iwoffii 6(3.79%). P. aeruginosa showed good sensitivity to Imipenem (97.95%), Piperacillin+Tazobactum (93.87%), cefoperazone (70.40%), Amikacin (68.38%) and Ticarcillin (64.28%). A .baumanii showed 100% sensitivity to Imipenem and 71.05% sensitivity to piperacillin+Tazobactum.

Conclusion: Psudomonas aeruginosa and A. baumannii were the common NFGNB isolated from the patients of Urinary tract infection (UTI), Long term care facilities (LTCF) and hospitalized patients, surgical site infection and ventilator associated pneumona. P. aeruginosa showed good sensitivity to imipenem, Piperacillin+Tazobactum, Cefoperazone, Moxifloxacin and Amikacin. While A. baumannii showed sensitivity to imipenem and piperacillin+Tazobactum.

NFGNB are exhibiting extensively resistance not only to beta lactam and the other groups of antibiotics but also to carbapenems. Emergence of resistance among organisms against the commonly used antibiotics is largely due to their lactamase production and indiscriminate use.

Keywords: Nonsocomial infection, Acinetobactor baumanii, imipenem, pseudomonas aeruginoa.