Abstract
Aim: bacterial infection with Mycobacterium tuberculosis is the main cause of tuberculosis. In recent year it has been seen that there were a tremendous advance in studies on the immunological diagnosis of tuberculosis. The main objective of the study To Evaluate role of Serum Immunoglobulin Levels as potential markers in tuberculosis diagnosis.
Method: Sixty patients were recruited as in this study who were diagnosed as tuberculosis patients from Patna Medical College and hospital at Patna. This diagnosis was done on the basis of patient history, clinical examination, sputum examination, chestradiography and related laboratory parameters. This patients were compared with fifty five healthy volunteers who were matched with age and sex. Blood sample were collected to measure the levels of IgA, IgM, IgE at hospital laboratory.
Result: When compared with normal healthy volunteers, levels of IgA, IgM, IgE were found to be increased significantly (p<0.001). There was significant increase in serum immunoglobulin levels observed among TB patients as compare to healthy volunteers.
Conclusion: May be due to humoral response to mycobacterial antigens, this Serum Immunoglobulin Levels were elevated in tuberculosis patients as compare to healthy volunteers.
References
- World Health Organization. 2010. [Accessed 20 April 2011]. http://www.emro.who.int/stb/pdf/CountryProfile-tun-08.pdf
- World Health Organization, “Treatment of tuberculosis: Guidelines for national programmes,” Geneva 2003, pp. 11-15.
- era-Cabrera L., Rendon A., Diaz-Rodriguez M., Handzel V., Laszlo A. 1999. Dot blot assay for detection of antidiacyltrehalose antibodies in tuberculous patients. Clin. Diagn. Lab 6:686–689.
- American Thoracic Society 2000. Diagnostic standards and classification of tuberculosis in adults and children. Am. J. Respir. Crit. Care Med. 161:1376–1395.
- K. Jain, H. S. Bishnoi, O. P. Beniwal, and S. N. Misra, “Immunoglobulin profile in pulmonary tuberculosis,” J Postgrad Med 1984, vol. 30, no. 80.
- P. Youmans, “Editorial-Relations between delayed hypersensitivity and immunity in tuberculosis,” Amer. Rev Resp. Dis., vol. 111, pp. 109-118, 1975.
- Casadevall A. Antibody-based therapies for emerging infectious diseases. Emerg Infect Dis 11016;2(3):200-8.
- Casadevall A, Pirofski LA. Antibody-mediated regulation of cellular immunity and the inflammatory response. Trends Immunol 2003;24(9):476-8.
- Casadevall A, Pirofski LA. A reappraisal of humoral immunity based on mechanisms of antibody-mediated protection against intracellular pathogens. Adv Immunol 2006;93:1-44.
- B. Selma, H. Harizi, and J. Boukadida, “Immunochromatographic IgG/IgM test for rapid diagnosis of active tuberculosis,” Clin Vaccine Immunol. 2011 December; vol. 18, no. 12, pp. 2090–2094.
- Julián, E., L. Matas, A. Hernández, J. Alcaide, and M. Luquin. 2000. Evaluation of a new serodiagnostic tuberculosis test based on the immunoglobulin A detection against Kp-90 Int. J. Tuberc. Lung Dis. 4:1082-1085.
- Daniel, T. M., and S. M. Debanne. 1987. The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay. Rev. Respir. Dis.135:1137-1151.
- Talat, B. M. Bhatti, and M. Yaqoob, “Comparative efficacy of different laboratory techniques used in diagnosis of tuberculosis in human population,” J. Medical Sci., vol. 2, pp. 137-144, 2002
- M. Fatolahzadeh, A. Bahador, H. Peeri-Dogaheh, M. Y. Alikhani, and R. Radmanesh- Ahsani, “Evaluation of different primer sets for the rapid diagnosis of tuberculosis,” Pak. J. Biol. Sci., vol. 10, pp. 107-111, 2007
- Geiger and P. Hoffman, “Quantitaive immunological determination of 16 different blood proteins in 260 normal children,” aged 0 to 15 years. Z. Kinderheilkd, vol. 109, pp. 22–40, 1970.
- T. Whicher, C. P. Price, and K. Spencer, “Immunonephelometric and immunoturbidometric assays for proteins,” Crit. Rev. Clin. Lab. Sci., vol. 18, pp. 213–260, 1983.
- J. Wittig, “Age related serum immunoglobulin E levels in healthy subjects and in patients with allergic disease,” J. Allergy Clin. Immunol., vol. 66, pp. 305–313, 1980.
- G.Johansson,H.H.Bennich,andT.Berg,“Theclinicalsignificance of IgE. Prog,” Clin. Immunol., vol. 1, pp. 157–181, 1972.
- Weiner J, Kaufmann SH. High-throughput and computational approaches for diagnostic and prognostic host tuberculosis biomarkers. Int J Infect Dis 2017; 56: 258–262.
- Wallis RS, Doherty TM, Onyebujoh P, et al. Biomarkers for tuberculosis disease activity, cure, and relapse. Lancet Infect Dis 2009; 9: 162–172.
- Goletti D, Petruccioli E, Joosten SA, et al. Tuberculosis biomarkers: from diagnosis to Infect Dis Rep 2016; 8: 6568.
- Petruccioli E, Scriba TJ, Petrone L, et al. Correlates of tuberculosis risk: predictive biomarkers for progression to active tuberculosis. EurRespir J 2016; 48: 1751–1763.
Corresponding Author
Dr Sunil Kumar Agrawal
Assistant Professor, Department of T.B. & Chest, Patna Medical College and Hospital, Patna, Bihar, India
DOI: https://dx.doi.org/10.18535/jmscr/v7i9.49
