Abstract
Background: Establishment of early prediction of oral cancer is important. We evaluated both lymphocyte DNA damage by Single cell gel electrophoresis (SCGE) and non-invasive urinary 8-hydroxy-2-deoxyguanosine (8-OHdG) biomarker of oxidative stress (OS). OS and poorly maintained antioxidant defense system may leads to increased intracellular reactive oxygen species (ROS) that can control basic cellular functions, such as proliferation and apoptosis which leads to the development of cancer.
Objectives: To evaluate total antioxidant status (TAS) and the extent of oxidative stress (OS) by measuring DNA damage in oral leukoplakia and oral squamous cell carcinoma (OSCC) in comparison to normal healthy individuals.
Methods: A total of 95 subjects aged 30 – 70 were included for the study. Of these 30 were healthy controls, 30 patients with oral leukoplakia and 35 clinically and histologically diagnosed patients with OSCC. Blood samples were evaluated for malondialdehyde (MDA), comet assay and antioxidants. Random urine specimens were collected for 8-OHdG estimation.
Results: Significant increase in DNA damage with decreased antioxidants status was observed in oral leukoplakia compared to OSCC patients and controls. TAS and lymphocyte DNA damage showed a strong negative correlation and comet assay and urinary 8-OHdG showed a strong positive correlation among three groups.
Conclusion: Oral cancer associated with OS causes genotoxic susceptibility in cancer. Lack of DNA repair mechanisms causes extensive DNA damage in oral leukoplakia patients, suggesting that OS is important in the pathogenesis of oral cancer.
Keywords: Antioxidants, comet assay, DNA damage, lymphocyte, oral cancer and oxidative stress.
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Corresponding Author
Dr. Subash. P
Associate Professor, Department of Biochemistry, KMCT Medical College, Kerala University of Health Sciences, Manassery 673602, Kozhikode, Kerala-India
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
DOI: https://dx.doi.org/10.18535/jmscr/v6i11.11
