Evaluation of Levels of Glycosylated Hemoglobin (HbA1c) in Patients with Type – 2 Diabetes Mellitus and in Healthy People, Attending P.M.C.H, Patna

Title: Evaluation of Levels of Glycosylated Hemoglobin (HbA1c) in Patients with Type – 2 Diabetes Mellitus and in Healthy People, Attending P.M.C.H, Patna

Authors: Dr Dilip Kumar, Dr S. Raza, Dr Satyendu Sagar

DOI: https://dx.doi.org/10.18535/jmscr/v6i10.44

Abstract

Objective: The aim of present study was observing the incidence and magnitude of abnormal concentrations of glycosylated hemoglobin (HbA_1C) in Type – 2 Diabetes Mellitus (NIDDM) and assessing the adequacy of control of the diabetic patients.

Materials and Methods: A total of 106 diabetic patients were selected in both sexes in different age groups, 20 healthy people were selected for control group, Glycosylated hemoglobin was measured by D-10, Column Chromatography methods(HPLC) supplied by Biorad. Blood sugar was measured by Hexokinase methods (Fully automated Roche Integra machine). Urine Sugar is measured by automated urine analyzer and also by rapid strip, supplied by Dirui Industries.

Results: Maximum incidence of DM occurred in the 5^th decade, there was male preponderance with male and female ratio of 4:1. The level of HbA_1c in untreated and treated diabetics was 8.30% + 0.37 (range 6.83% - 10.40%) and 7.72% + 0.44 range (5.52% -12.41%). The corresponding FBS levels were 181 + 17 mg/dl (range 130 – 282 mg/dl) and 151 + 17 mg/dl (range 78 – 310 mg/dl) respectively. The mean of glycosylated Hemoglobin in normal individuals was found to be 5.30% + .24 (range 4.50% - 6.5%) and mean FBS level was 79 +2mg/dl (range 68 – 88 mg/dl).The value of HbA_1c was approximately 1.5 times higher in untreated diabetics then in the control group. This was statistically significant. The HbA_1c level correlated closely with the FBS level in diabetics.

Conclusion: It is concluded that in assessing glycemic control in diabetics with high glycosylated hemoglobin levels, concurrent FBS levels estimations are essential. However, estimation of HbA_1c should be viewed as an adjunct to and not a replacement for FBS estimation in the assessment of glycemic control in treated diabetics.

Keywords: Diabetes mellitus, Glycosylated Hemoglobin, fasting blood glucose level.

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