Title: Comparative Study of commonly sexually transmitted disease (Syphilis and gonorrhoea) in the Dhanbad District of Jharkhand
Authors: Dr Sujeet Kumar Tiwary, Dr Siyavar Sharan, Dr Binay Kumar Singh, Dr Sunil Kumar Sinha, Dr Rajendra Kumar
DOI: https://dx.doi.org/10.18535/jmscr/v6i7.139
Abstract
Sexually transmitted disease which spreads mainly by sex with infected persons either by Anal and oral sex. More than 30 different Types of bacteria, viruses and parasites can be transmitted through sexual activity but most commonly is syphilis and gonorrhoea.[1,2] Syphilis is caused by the Treponema pallidum Bacteria .syphilis is classified into three stages- primary, secondary ,tertiary .After patients acquired infection; patients suffer from many serious Health problems ,if it is not treated Properly and Timely ,Eventually, the Patients with stage of tertiary syphilis will die.[3,4] Second most common infection of STD is Gonorrhoea, which is caused by the Neisseria gonorrhoea. Neisseria Gonorrhoea is spread through sexual contact with an infected person.[5, 6]
Materials and Method: This study carried out from 28 April 2016 to 29 April 2018 in the Patliputra Medical College & Hospital Dhanbad. A total of 700 samples were collected from the STD suspected patients, with or without any complaints as enunciated by WHO in its syndromic approach for the diagnosis of STI (WHO, 2001 and Choudhry, et al 2010) were includes as subjects. Three hundred (230 Males and 70 Females) examined for syphilis, while four hundred (280 males and 120 Females) examined for gonorrhoea .followed up patients were excluded from the study. Detailed history and clinical features were recoded from all the patients. All samples were screened for common STIs, by standard microbiological Methods.
Result: In this comparative study we found the total number of gonorrhoea positive male patients 95 and female 35, overall total gonorrhoea positive 130, and the total syphilis positive male 28 and female 34, over all syphilis positive 62, we are found this gonorrhoea infection is more than higher for syphilis.
Laboratory identification of Syphilis and Gonorrhoea: All suspected patients both Male and female Urethral Swab, High Vaginal swab and Endocervical Swab were collected in the Department of microbiology of Patliputra Medical college and Hospital. Direct examination with use of wet preparation method. Gram staining method was carried out on both high vaginal swab (HVS) and Endocervical swab (ECS) and examined 100x objective under oil immersion and identified Gram negative diplococci shape gonorrhoea. Endocervical swab specimens were inoculated into blood agar and Thayer Martin agar while High vaginal swab specimen were inoculated into blood agar. After 48 hours all inoculated specimens examine direct microscopy in the help of grams stains and identified diplococci Shape gonorrhoea.
Serological test procedure use for diagnosis of syphilis , From All patients 3-5 ml of Blood samples were collected in a sterile plastic containers and separated serum from all samples and used within two days for screening tests of syphilis .Other were frozen for confirmatory tests. Syphilis antibodies were tested for using syphilis ultra – rapid tests strips.
Data analysis: All Data were analysed with standard statistical method, Percentage were computed for all variables. Result were presented in the form of table & column.
Keyword: Gonorrhoea, Syphilis, Infection, Mucous, Neurological.