Abstract
Detection of ESBLs producing organisms from samples such as urine represents an epidemiologic marker of colonization. Thus the present study was aimed to know the antimicrobial resistance pattern among ESBL producing urinary isolates of E. coli which will helps in deciding empiric antibiotic therapy and identifying measures to reduce increasing resistance trends. 106 consecutive non repeating uropathogenic E. coli included in the study. All specimens were cultured using a calibrated loop. Identification of E. coli was done by standard biochemical tests. Antimicrobial susceptibility testing was done by Kirby-Bauer’s disc diffusion method, isolates were screened for ESBLs production and confirmation by combination disk method as per CLSI 2014 guidelines. E. coli ATCC 25922 and Klebsiella pneumonia ATCC 700603 were used as a negative control and positive control respectively. Out of 106 uropathogenic E. Coli, 99(93.4%) strains identified by ESBL screening and 48 (45.2%) were confirmed for ESBLs production. Minimum resistance to Imipenem (1%) followed by Meropenem (2%) and Nitrofurantoin (4%), and maximum resistance to Piperacillin(100%) followed by Cefipime (96%), Ciprofloxacin (96%) and Norfloxacin (91%) were seen. Thus Imipenem followed by Nitrofurantoin and Amikacin remains the drug of choice for ESBL producers with the highest sensitivity depending on severity and risk factors.
Keywords: HAI, UTI, beta-lactamase.
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Corresponding Author
Dr Manish Purohit
Associate Prof, Microbiology, MGM Medical College, Indore
Mob: 8109671170, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
DOI: https://dx.doi.org/10.18535/jmscr/v6i4.131
