Abstract
Objectives & Aims: 1) To determine the hematological findings & coagulation profile of the study subjects. 2) To find out relevant clinical findings of study subjects through clinical examination & detail history. 3) To find the correlation of the clinical findings with the hematological findings studied. To establish probable diagnosis in the study subjects. To find the incidence of spectrum of diseases in bleeding disorders. To find the age and sex distribution of the cases studied.
Material & Methods: Blood was collected in a sterile EDTA containing tube and processed following our established hospital based laboratory protocol. A complete blood counting including HB%, PCV, Red cell indices, platelet count, total white cell count done by Automated blood cell counter. The all cell count indices including RBC, WBC count with differential along with morphological changes further confirmed by manual oil immersion smear study method. Peripheral smears study was done with field A and B stain and leishman stain.
Conclusion: This In our study we found that in most of the cases of thrombocytopenia i.e below 1,50000 count (81%), automated counter give very low platelet counts while on peripheral smear examination, the count is not that much reduced but different morphological variations of platelets like megathrombocytes, platelet aggregates and platelet fragments are found. These variations denote inactive or non functional platelets, hence despite of the low normal or near normal platelet counts , patient present with bleeding.
References
1. Acute immune thrombocytopenia (ITP) in childhood: retrospective and prospective survey in Germany ;Sutor AH, Harms A
- Aetiological considerations of acquired aplastic Anaemia; Saqib Malik, Iram Sarwar
- Age and the Prevalence of Bleeding Disorders in Women With Menorrhagia ;Philipp, Claire S
- Allen GA, Gladers B. Approach to the bleeding child. Ped Clin North Am
- Amorosi EL, Ultmann JE (1966). "Thrombocytopic purpura: report of 16 cases and review of the literature". Medicine (Baltimore) 45: 139–159.
- Angela M. Cheung et al. (2008), Vitamin K Supplementation in Postmenopausal Women with Osteopenia (ECKO Trial): A Randomized Controlled Trial, Bunyaratavej N (2007). "[Experience of vitamin K2 in Thailand]" (in Japanese). Clin Calcium 17 (11): 1752–60.
- Annexin II and Bleeding in Acute Promyelocytic Leukemia ;Jill S. Menell, Gabriela
- BESSMAN, J. D. (1986) Automated Blood counts & differentials .A practical guide. John Hopkins University Press, Baltimore.
- Biggs R, MacFarlane RG. Hemophilia & related conditions: survey of 187 cases
- Bleeding disorders: A common cause of menorrhagia in adolescents Jennifer A. Bevan
- Bleeding manifestations in severely thrombocytopenic children with immune thrombocytopenic purpura ;Chandra J, Ravi R
- Diacovo T.G. et al. (1996). "Platelet-mediated lymphocyte delivery to high endothelial venules". Science 273 (5272): 252–5. doi:10.1126/science.273.5272.252. PMID 8662511
- Doctor Cecil Kaplan (2003-11-01). "Fetal and Neonatal Alloimmune Thrombocy-topenia". Orphanet Encyclopedia.
- Epidemiological features of aplastic anaemia in Pakistan ;Adil SN, Burney IA
- Erpenbeck L, Schön MP (April 2010). "Deadly allies: the fatal interplay between platelets and metastasizing cancer cells". Blood 115 (17): 3427–36.
- Furie B, Furie BC (2005). "Thrombus formation in vivo". J. Clin. Invest. 115 (12): 3355–62. doi:10.1172/JCI26987. PMC 1297262. PMID 16322780. Boekhout Mussert MJ, Vander Kolk Schaap PJ, Hermans J, Loelijer EA. Tripodi A, Chatarangkul V, Braga M, et al. Results of multicentre study assessing the status of a recombinant thromboplastin.
- Bowen DJ: Haemophilia A and haemophilia B: molecular insights. Mol Pathol 2002; 55:1
- Bowie, EJW et al. Mayo clinic laboratory manual of hemostasis, Philadelphia; W.B. Saunders and Co., 1971; 29-33.
- Campbell, Neil A. (2008) Biology (8th ed.). London: Pearson Education.p. 912..
- Chromosomal Breakage Study in Aplastic Anemia Patients in India :1D. Jain
- Clinical audit of inherited bleeding disorders in a developing country ;Sajid R, Khalid
- Davidson, Stanley; Haslett, C. (2002). Davidson's Principles and Practice of Medicine (19 ed.). Edinburgh: Churchill Livingstone. ISBN 0-443-07036-9.
Corresponding Author
Dr Shailendra Singh Thakur
Email- This email address is being protected from spambots. You need JavaScript enabled to view it.
DOI: https://dx.doi.org/10.18535/jmscr/v6i4.04
