Abstract
Background: Culture is the gold standard for diagnosis of enteric fever. Due to the poor isolations of Salmonella in recent times, Widal test is still a useful alternative aid. Good interpretation of the test cannot be made unless we know the correct baseline titres in population of particular area.
Aim and Objectives: To determine the base line titres of antibodies in healthy individuals.
Material and Methods: Blood samples were collected from 100 healthy individuals from August to November 2017. Widal tube agglutination test was performed to know the titres.
Results: In the total samples screened, most frequently recorded titre for O & H agglutinins were 1:40 for Salmonella Typhi. Similarly for Salmonella Paratyphi A and B, anti H titres were 1:20 each respectively.
Conclusion: It is concluded that the cut-off value of antibodies titres for Salmonella Typhi O and H antigens is >1:40, and for Paratyphi A & B H antigen is >1:20 in this region. Any titre more than this should be taken as high and interpreted in correlation with clinical findings or titers observed in paired sera.
Keywords: Baseline titre, Widal tube agglutination test, Enteric fever, Healthy population.
References
- Abdul Razak SH, Abbas Abood AD, Adawia Fadil A. The distribution of anti –salmonella antibodies in the sera of healthy blood donors in baquba city. irqui j comm.med 2011; 24: 241-44.
- Deepak P, Komal Raj R, Bimala S, Suresh Raj K, Bishnu Raj T. baseline titre and diagnostic cut off value for widal test: A comparative study in healthy blood donors and clinically suspected of enteric fever. JHAS 2012; 2:22-26.
- Crump JA, Luby Sp, Minz ED. The global burden of typhoid fever. Bulletin world health organization 2004; 82:346-53.
- Pang T, Puthucherry SD. The significance and the value of the Widal test in the diagnosis of typhoid fever in an endemic area. J Clin Pathol. 1983;36:471–75.
- Patil AM, Kulkarni ML, Kulkarni AM. Baseline Widal Titres in Healthy Children. Indian J. of Peadiatr. 2007;74:1081–83.
- Gilles RR. Medical Microbiology. 12th edition. AP314. Edinburg: Churchill Livingstone; Salmonella : The Widal test. In Cruickshank R, Dugid JP, Marmion BP, Swain RH. eds.
- Punia JN, Joshi RM, Gupta V, Arora RK. Determination of the baseline Widal titres from Chandigarh. Indian J. Med. Microbiol. 2003;21(2):144.
- Bharat MP, Rajendra K, Shyam KM, Janak K. Distribution of antibody titre against Salmonella enterica among healthy individuals in Nepal. Annals of Clin Microbiol anti-microbial. 2009;8:112–18.
- Peshattiwar,P.2012.Study of the baseline Widal titre amongst healthy individuals in Amlapuram,India.J.Clin.Diag.Res.,6(3);416-417.
- Saxena, N., Maheawari, D.and Dadhich, D. 2012. Baseline Widal titres among apparently healthy individuals in Hadoti region,Rajasthan.J.evol.Med.Dent.Sci.,2(15):2425-29.
- Shekhar Pal, The Baseline Widal Titre Among the Healthy Individuals of the Hilly Areas in the Garhwal Region of Uttarakhand, India, Journal of Clinical and Diagnostic Research.2013 March, Vol-7(3):437-440.
- Jeyakumari et al., Study Of The Baseline Widal Titres Among Healthy Individuals of Rural Population In Pondicherry. Int J Med Res Health Sci.2015;4(2):322-26.
- Sunil Sonu Hatkar, Baseline widal titre among the healthy individuals: a study in and around Badnapur, Marathwada region at Tertiary Care Hospital, Indian J Microbiol Res 2016;3(2):162-165 162.
- Nidhi Sharma et al, Study of the Baseline Widal Titres among Healthy Population in a Tertiary Care Hospital in Central India JMSCR Volume 05 Issue 04 April 2017.
Corresponding Author
Dr CH. Aruna Kumari
Department of Microbiology, Rangaraya Medical College, Kakinada, A.P
DOI: https://dx.doi.org/10.18535/jmscr/v6i2.107
