Abstract
Background: Immunohistochemistry provides a true identitiy for undifferentiated tumours. The accurate typing of malignant and benign tumours are important for prognostic and therapeutic purposes.
Aim: The present study is designed
- To identify the histogenesis of undifferentiated malignant tumours by immunohistochemical analysis.
- To take out spectrum of tumours diagnosed as undifferentiated malignant, poorly differentiated and small round cell on histology.
Materials & Methods: A total of 92 cases diagnosed as undifferentiated malignant tumours, poorly differentiated malignant tumours as well as small round cell tumours on histopathology in the Department of Pathology, SMSMC Jaipur from October 2015 to January 2017 were taken and immunohistochemistry was applied to reach the final diagnosis.
Results: Its was possible to arrive at final diagnosis in 89 (96.73%) cases with the help of immunohistochemistry. Majority of the cases were of Mesenchymal lineage, among them Ewing’s sarcoma/PNET was the most frequent diagnosis followed by Non Hodgkin’s lymphoma (DLBCL) in hematopoietic lineage
Conclusion: Immunohistochemistry is valuable adjunct to H & E staining in diagnosis of undifferentiated tumours. Accurate typing is prognostically and therapeutically important, for that a panel approach of carefully selected antibodies is always recommended.
Keywords: Immunohistochemistry, Undifferentiated Malignant tumours, Small round cell tumours.
References
- Introduction to tumor biology by I. De Wever, differentiation and heterogeneity in tumors R. sciot: 61-62
- Coons A, creech jj, jones RN, Berlinger E.the demonstrations of pneumococcal antigen in tissue by the use of fluorescent antibody. J Immunol 1942;45:159-70.
- Taylor CR. The current role of immuno-histochemistry in diagnostic pathology. Adv Pathol Lab Med. 1994;7:59
- Taylor CR. An exaltation of experts: Concerted efforts in the standardization of immunohistochemistry. Hum Pathol. 1994;25:2.
- Taylor CR, Cote RJ, eds. Immuno-microscopy: A Diagnostic Tool for the Surgical Pathologist. 2nd ed. Philadelphia: WB Saunders; 1994.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin containing cells in formalin-fixed, paraffin- embedded tissues using peroxidase labeled antibody. J Clin Pathol. 1974;27:14
- Slapak CA, Kufe DW. Principles of cancer therapy. In Isselbacher KJ, Braunvald E, Wilson JD, Martin JB, Fauci AS, Kasper DL eds: Harrison’s Principles of Internal Medicine, 13th Edition. Vol 2, 1994; McGraw-Hill, Inc. 1826-1840.
- Bahrami A, Truong LD, Ro JY. Undifferentiated tumor: true identity by immunohistology. Arch Pathol Lab Med. 2008;132:326-48.
- Ahmed Z, Azad NS, Bhurgari Y, Ahmed R, Kayani N, Pervez S et al. Significance of immunohistochemistry in accurate characterization of malignant tumors. J Ayub Med Coll Abbottabad. 2006;18:38-43.
- Taylor CR, Shi SR, Barr NJ, Wu N. Techniques of immunohistochemistry: principles, pitfalls and standardization. In: Dabbs DJ, editor. Diagnostic Immuno-histochemistry, 2nd ed. Philadelphia: Churchill Livingstone Elsevier Inc.; 2006. p. 1-42.
- Williams JH, Mepham BL, Wright DH. Tissue preparation for immunocyto-chemistry. J Clin Pathol. 1997;50:422-8.
- Coindre JM, Tanguy F, Merlio JP, De Mascarel I, De Mascarel A, Trojani M. The value of immunohistological techniques in undifferentiated cancers. Tumori. 1986;72;539-44.
- Vege DS, Soman CS, Joshi UA, Ganesh B, Yadav JN. Undifferentiated tumors:an immunohistochemical analysis on biopsies. J Surg Oncol. 1994;57:273–276.
- Bashyal R1 , Pathak TB1 , Shrestha S1 , Pun CB1 , Banstola S1 , Neupane S1 , Lee MC1. Role of immunohistochemistry in the diagnosis of malignant small round cell tumors. Journal of Pathology of Nepal (2011) Vol. 1, 87-91
- Pity IS. Histopathological and immuno-histochemical approach for characte-rization of undifferentiated malignant tumors. JABHS. 2011;12:49-57.
- Vasudha Bhagat, Amita Patel, Jigna Modi, Peeyush Saini, Hemali Tailor, Kumar bhargav Kaptan, immunohistochemistry: a diagnostic tool in Accurate characterization of undifferentiated malignant tumors, International Journal of Medical Science and Public Health | 2013 | Vol 2 | Issue 4, 921-926
- Bianchini WA, Altemani AM, Paschoal JR. Undifferentiated head and neck tumors: the contribution of immunohistochemical technique to differential diagnosis. Sao Paulo Med J. 2003;121:244-7.
Corresponding Author
Dr Praveen Singh Dhakar
C-39, Resident Doctors Hostel,
SMS Medical College, Jaipur- 302004 INDIA
Mob-9694443292, 7976569885, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
DOI: https://dx.doi.org/10.18535/jmscr/v6i2.09
