Abstract
Introduction: Use of single donor platelets (SDP) has increased by radical change over past few years. SDP not only increase the quality of product in terms of decreased cellular contamination but also increases the overall yield of platelet collected. However various donor parameter exert their effect on yield of single donor platelet apheresis.
Materials and Methods: The present study included the outcome of 65 SDPA procedures that were conducted on Haemonetics MCS+ intermittent flow cell separator (Haemonetic Corporation). It employs single venous access utilizing closed collection apheresis kits with efficient leukoreduction. Study was undertaken at blood bank M.Y. Hospital associated with M.G.M. Medical College, Indore, and a tertiary care Central Government Teaching Institute.
Results: The mean platelet yield was 2.7± 0.83(1.6-4.9 × 1011/µl).Platelet yield showed high statistical significance with pre donation platelet count (p =0.000). Other than pre donation platelet counts, SDP yield also showed statistical significant correlation with weight and MPV. However no statistical significance was seen height (p=0.838), Hemoglobin (p=0.554) pre donation WBC count (p=0.052) and PDW (p=0.963) of donor on platelet yield.
Conclusion: Hence, in our study, we found platelet yield showed positive statistical significance with pre donation platelet count as well as with pre donation weight and MPV. To find out interventions that could increase pre donation platelet would have a positive effect on platelet product yields
Keywords: Apheresis, Platelet yield, Single donor platelet (SDP), Parameters.
References
- Guide to the preparation, use and quality assurance of blood components European committee on Blood Transfusion. 2010-16 th Ed.
- Kakaiya R, Aaronson C, Julleis J. Whole blood collection and component processing. Technical Manual AABB.16 th edition. Bethesda: AABB; 2008.189-228.
- Guerrero-Rivera S, Gutierrez-Espindola G, Talavera JO, Meillon- Garcia LA, Pedraza-Echevarria M, Pizzuto-Chavez J. Hemoglobin and platelet count effect on platelet yields in plateletpheresis. Arch Med Res. 2003; 34:120–22.
- Katz L, Palmer K, McDonnell E, Kabat A. Frequent plateletpheresis does not clinically significantly decrease platelet counts in donors. Transfusion.2000; 47(9):1601–06.
- Strauss RG. Risks of clinically significant thrombocytopenia and/or lymphocy-topenia in donors after multiple plateletpheresis collections. Transfusion. 2008;48:1274-78.
- Saran RK. Apheresis. Transfusion Medicine Technical Manual. New Delhi. Directorate General of Health Services, Ministry of Health & Family Welfare, 2nd ed. Government of India 2003:229-43
- Goodnough LT, Kuter D, McCollough J, Brecher ME. Apheresis platelets: emerging issues related to donor platelet count, apheresis platelet yield, and platelet transfusion dose. J Clin Apheresis. 1998; 13:114–19.
- Das SS, Chaudhary RK, Shukla JS. Factors influencing yield ofplateletpheresis using intermittent flow cell separator. Clin Lab Haematol. 2005;27:316-19.
- Chaudhary R, Das SS, Khetan D, Sinha P. Effect of donor variables on yield in single donor plateletpheresis by continuous flow cell separator. Transfus Apher Sci. 2006 Apr; 34(2):157-61.
- Shalini Bahadur et al., Apheresis Platelets: A Study of Effect of Donor Variables on Outcome of Plateletpheresis.National Journal of Laboratory Medicine. 2015; 8829:000.
- Tyler V, Brecher M, Pisciotto P, Pierce S, editors. Technical American Association of Blood Banks Manual, 13th ed. Bethesda: Maryland, USA. 1999;p. 1-798.,
- Recommendation No. R (95) 15. Guide to the preparation, use and quality assurance of blood components 7th ed Strasbourg, France: European Council Press. 2001; p 123
- Saran, R. K. (2003) Apheresis. In: Transfusion Medicine Technical Manual. Ministry of Health and Family Welfare, Govt. of India, New Delhi, India.pp.229-44.
- Hester JP, Ventura GJ, Boucher T. Platelet concentrate collection in a dual stage channel using computer generated algorithms for collection and prediction of yield. Plasma Ther Transfus Technol. 1987; 8:377–85.
- Buchholz DH, Squires JE, Herman JH, Ng AT, Anderson JK, Hedberg SL. Plateletpheresis in 90 to 110 pound donors using the CS3000 blood cell separator. Transfusion. 1997; 37:715–18.
Corresponding Author
Dr Amrita Tripathi
Add.- G-133 Parth Avenue App., Shivshakti Nagar,
Bicholi Hupsi Road, Indore (M.P), India
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DOI: https://dx.doi.org/10.18535/jmscr/v6i1.183
