Abstract
Objectives: To study the microbiological profile of neonatal septicemia and to determine the anti-microbial resistance pattern of isolated bacteria.
Study Design: retrospective analysis of blood culture reports of neonatal septicemia cases.
Setting: tertiary level neonatal intensive care unit
Patients and Methods: case records of neonatal septicemia cases were retrieved and their culture results were analysed in terms of causative organisms and their antimicrobial sensitivity patterns. Extended spectrum beta lactamamse (ESBL) resistance and methicillin resistant Staphylococcus Aureus (MRSA) patterns were studied.
Results: out of 162 samples, bacterial growth was detected in 53 samples. Gram negative growth was around 66 percent and rest were gram positive. Gram negative organisms accounted for more than 75 percent in both early and late onset sepsis. Out of 12 blood cultures showing Staph Aureus, 7 (58%) were MRSA. Vancomycin was the drug to which all MRSA growths were sensitive. Out of 34 gram negative infections, 13 were ESBL in whom Klebsiella sp. was the most common followed by E. coli. All ESBL organisms were sensitive to Imipenem.
Conclusion: gram negative organisms predominated in the neonatal septicemia. MRSA and ESBL strains hold a large share of the bacterial growths.
References
- Chandel DS, Johnson JA, Chaudhry R, Sharma N, Shinkre N, Parida S, et al. extended-spectrum B-lactamase producing gram negative bacteria causing neonatal sepsis in india in rural and urban settings. J med microbial.2011;60(4) ;500-7.
- Jyothi P, Basavaraj MC, Basavaraj P V. bacteriological of neonatal septicaemia and antibiotics susceptibility pattern of isolates. J Nat Sci Biol Med [internet]2013;4(2):306-9. Available from: http://www.pubmedcentral.nih.gov/articlerender.fegi?artid=3783770&to01=pmcentrez&rendertype=abstract accessed on june 30,2017
- Donald Waters, Issrah Jawad, Aziez Ahmad, Ivana Lksic, Harish Nair, Lina Zgaga, Evropi Theodoratou, Igor Rudan, Anita K.M. Zaidi, and HC. Aetiology of community acquired neonatal sepsis in low and middle income countries. J Globe Heal [internet].Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484773/ accessed on june 30,2017
- Ann L Anderson-BerryAnn L Anderson-Berry, MD,PhD Associate Professor of Pediatrics, Section of Newborn Medicine, University of Nebraska Medical Centre, Creighton University School of Medicine; Medical Director NICU NMC, Ann L Anderson-Berry MD, PhD is a member of following medical societies: American Academy of Pediatrics, Nebraska Medical Associatio S for PR, Disclose.DN to co-author(s), Linda L Bellig, MA RN NNP, (Retired) Track Coordinator, Instructor, Neonatal nurse Practitioner Program MU of SSC of N, Disclose. DN to, et al. Neonatal sepsis.2015; available from: http://emedicine.medscape.com/article/978352-overview#a4 accessed on june 30,2017
- Peirovifar A, Ahangar M, Gharehbaghi MM. Prevalence of multidrug Resistant Extended-spectrum Beta-lactamse producing Gram-Negative Bacteria in Neonatal Sepsis Introduction: Int J Women`s Heal Reprod Sci.2014;2(3)
- Nachhatarjitsingh, pavneetkaur, aruna Aggarwal. Neonatal septicaemia bt beta-lactamases producing multi-resistant organism-cause of concern.Int J basic Sci[internet].2016;7(1). Available from: http://www.ijbms.com/micro-biology/neonatal-septicemia-by-beta-lactamse-producing-multiresistant-organism-cause-of-concern-nachhatarjit-singh-pavneet-kaur-aruna-aggarwal/ accessed on june 30,2017
- Roy 11, Jain A, kumar M AS. Bacteriology of neonatal septisemia in a tertiary care hospital of northern india. Indian J Med Microbiol [Internet]. 2002;20(3):156-9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/17650757. accessed on june 30,2017
- Kayange N, Kamugisha E, Mwizamholya DL, Jeremiah S, Mshana SE. Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in tertiary hospital, Mwanza-Tanzania.2010.
- Mohammad S, Aletayeb H, Khosravi AD, Dehdashtian M, Kompani F, Mortazavi SM, et al. Identification of bacterial agents and antimicrobial susceptibility of neonatal sepsis: A 54-month study in tertiary hospital. African J Microbiol Res. 2011;5(5):528-31
- A Bhattacharjee, MR Sen, P Praksh, A Gaur SA. Increased prevalence of Exten-ded Spectrum B Lactamase Producers in Neonatal Septicemic Cases At a Tertiary Referral Hospital. Indian J Med Microbiol [internet]2008;26(4):356-60.
- Arora U, Devi P. Bacterial profile of blood stream infections and antibiotic resistance pattern of isolates. JK Sci.2007;9(4):186-90
- Dhara M, Disha P, Sachin P, Manisha J, Seema B, Vegad MM. Comparison of Various Methods For The Detection Of Extended Spectrum Beta Lactamase In Klebsiella Pneumoniae Isolated From Neonatal Intensive Care Unit. Natl J Med Res.2012;2(3):348-53.
- Mustafa M, Ahmed SL. Original article Bacteriological profile and antibiotic susceptibility pattern in neonatal septicaemia in view of emerging drug resistance. J Med Allied Sci.2014;4(1):2-8.
- Samaga MP, Sumangala B. Bacteriolo-gical profile of neonatal septicaemia in MIMS, Mandya, India. Int J Curr Microbiol Appl Sci.2016;5(3):495-501.
- Marwah P, Chawla D, Chander J, Guglani V, Marwah A. Bacteriological profile of neonatal sepsis in tertiary care hospital of north India. Indian Pediatr. 2015;52[2]: 158-9.
- Gupt PK, Patra DP. Acinetobacter sepsis in new born. Indian Pediatr. 1998;35:27-32.
- Kamble R, Ovhal R. Original research article Bacteriological Profile of Neonatal Septisemia. Int J Curr Microbiol Appl Sci. 2015;4(2):172-82
- Chelliah A, Ravinder T, Katragadda R, Leela KV, Narayana Bahu R. isolation of MRSA, ESBL and AmpC-B –Lactamses from neonatal sepsis at a tertiary care hospital. J Clin Diagnostic Res.2014;8 (6):24-7.
- Kaistha N, Mehta M, Singla N, Garg R, Chander J. Letter to the Editor Neonatal septicaemia isolates and resistance patterns in a tertiary care hospital of North India. J Infect Dev ctries.2007;4(1):55-7.
- Pandey S, Raza MS, Bhatta CP. Preval-ence and Antibiotic Sensitivity Pattern of Methicillin-Resistant-Staphylococcus aureus in Kathmandu Medical College –Teaching Hospital. J Inst Med.2012;34 (1):13-7.
- Saravanan M, Nanda A, Tesfaye T. antibiotic Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus from Septicaemia Suspected Children in Tertiary Hospital in Hosur, South India. Am J Microbiol Res [internet].2013;1(2):21-4.
- Subitha B, Selvan BS. Plasmid mediated AmpC beta lactamase producing gram negative isolates in neonatal septicaemia. Int J Curr Microbiol Applsci [internet].2015;4(8):391-8 Available from: http:// ijcmas.com/vol-4-8/B.senthil selvan.pdf accessed on june 30,2017
- Roy S, Gaind R, Chellani H, Mohanty S, Datta S, Singh AK, et al. Neonatal septoicemia caused by divers clones of Klebsiella pneumonia & Escherichia coli harbouring blaCTX-M-15. Indian J Med Res.2013;137(4):791-9.
- Premalatha DE, Koppad M, Halesh LH, Siddesh KC, Prakash N. The Bacterial Profile and Antibiogram of Neonatal Septicemia in a Tertiary Care Hospital. Int J Recent Trends Sci Technol.2014; 110(3):451-5.
- Thakur S, Thakur K, Sood A, Chaudhary S. Bacteriological profile and antibiotic sensitivity pattern in neonatal septicaemia in a rural tertiary care hospital in North India. Indian j Med Microbiol.2016; 9(4):67-71.
- Mane AK, Nagdeo N V, Thombare VR. Study of neonatal septicaemia in a tertiary care hospital in rural Nagpur. J Recent AdvAppl Sci.2010;25:19-24.
- Gandhi S, Ranjan K, Ranjan N, Sapre N, Masani m. Incidence of neonatal sepsis in tertiary care hospital: An overview. Int J Med Sci Public Heal [internet]. 2013;2(3):522 Available from: http;//www.scopemed.org/?mno=34358. accessed on june 30,2017
- Shehab El-Din EMR, El-Sokkary MMA, Bassiouny MR, Hassan R. epidemiology of Neonatal Sepsis and Implicated Pathogens: A Study from Egypt. Biomed Res Int [internet]. 2015;2015:1-11. Available from: ttp:/www.hindawi.com/journals/bmri/2015/509484/ accessed on june 30,2017
- Shrestha, N.J.Subedi, K.U.Rai GK. Bacteriological profile of neonatal sepsis: a hospital based study. J Nepal paediatr Soc 2011.2011;31(1):1-5.
- Khinchi Y, Kumar A, Yadav S. Profile of Neonatal sepsis. J Coll Med Sci.2010;6(2):1-6.
- Shrestha P, Das B, Bhatta N, Jha D, Setia A, et al. Clinical and Bacteriological profiles of blood culture Positive Sepsis in Newborns. Vol.27, Journal of Nepal Paediatric Society.2009.p.64-7.
Corresponding Author
Dr Baljeet Maini
Professor, Department of Pediatrics, MMIMSR, Mullana
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
DOI: https://dx.doi.org/10.18535/jmscr/v5i11.158
