Enterococcus hirae, Unexpected Bacteria Detected in Rectal Swab of A Subject in Nduga District, Papua Province by 16s rDNA Sequencing System

Title: Enterococcus hirae, Unexpected Bacteria Detected in Rectal Swab of A Subject in Nduga District, Papua Province by 16s rDNA Sequencing System

Authors: Hotma Martogi Lorensi Hutapea, Ratna Tanjung, Vatim Dwi Cahyani, Yustinus Maladan, Hana Krismawati, Antonius Oktavian

DOI: https://dx.doi.org/10.18535/jmscr/v5i5.160

Abstract

Enterococci are opportunistic pathogens known as a significant cause of nosocomial and community-acquired infections. Enterococcus faecalisand E. faeciumare the most common species identified in this infection. Another species, E. hirae is known to infect animals, and rarely isolated from human. The goal of this study was to characterize Enterococci derived a4 years old female rectal swab. The characterization was performed using MicroSeqID^TM500 sequencing system. DNA genomic was extracted from single colony and characterized using MicroSeqID^TM500 system. The sequence data was analyzed by homology analysis to MicroSeq library^TM, genbank, and phylogenetic. The sequencing results showed that the isolate highly homolog to Enterococci sp. Phylogenetic analysis of 16S rDNA showed that the isolate was closely related to Enterococcus hirae.

Keywords: Enterococci, Enterococcus hirae, MicroseqID^TM500, 16S rDNA.

References

1. Dicpinigaitis P V, Aguirre M De, Divito J. Enterococcus hirae Bacteremia Associated with Acute Pancreatitis and Septic Shock. Hindawi. 2015:5-8. doi:10.1155/2015/123852.

2. Devriese LA, Vancanneyt M, Descheemaeker P, et al. Differentiation and Identication of Enterococcus durans , E. hirae and E. villorum. J Appl Microbiol. 2002;92:821-827.

3. Janda JM, Abbott SL. 16S rRNA Gene Sequencing for Bacterial Identification in The Diagnostic Laboratory: Pluses, perils, and pitfalls. J Clin Microbiol. 2007;45(9):2761-2764. doi:10.1128/JCM.01228-07.

4. Woo PCY, Ng KHL, Lau SKP, et al. Usefulness of the MicroSeq 500 16S Ribosomal DNA-Based Bacterial Identification System for Identification of Clinically Significant Bacterial Isolates with Ambiguous Biochemical Profiles. J Clin Microbiol. 2003;41(5):1996-2001. doi:10.1128/JCM.41.5.1996-2001.2003.

5. Tim Gerak Cepat Kementerian Kesehatan Republik Indonesia. Laporan Verifikasi Kasus Kematian Balita Oleh Sebab Yang Belum Diketahui Di Kecamatan Mbua Kabupaten Nduga Provinsi Papua.; 2015.

6. Fisher K, Phillips C. The ecology , epidemiology and virulence of Enterococcus. 2016;(2009):1749-1757. doi:10.1099/mic.0.026385-0.

7. Bourafa N, Loucif L, Boutefnouchet N. Enterococcus hirae , An Unusual Pathogen in Humans Causing Urinary Tract Infection in A Patient with Benign Prostatic Hyperplasia: First Case Report in Algeria. New Microbes New Infect. 2015;8:7-9. doi:10.1016/j.nmni.2015.08.003.

8. Etheridge ME, Yolken RH, Vonderfecht SL. Enterococcus hirae Implicated as a Cause of Diarrhea in Suckling Rats. J Clin Microbiol. 1988;26(9):1741-1744.

9. Pãosinho A, Azevedo T, Alves J V, et al. Case Report Acute Pyelonephritis with Bacteremia Caused by Enterococcus hirae : A Rare Infection in Humans. 2016;2016:10-13.

10. Savini V, Bonfini T, Marrollo R, et al. Enterococcus hirae: A Zoonotic Microorganism in Human Umbilical Cord Blood. World J Microbiol Biotechnol. 2013. doi:10.1007/s11274-013-1537-4.

11. Talarmin JP, Pineau S, Guillouzouic A, et al. Relapse of Enterococcus hirae Prosthetic Valve Endocarditis. J Clin Microbiol. 2011;49(3):1182-1184. doi:10.1128/JCM.02049-10.

12. Franz CMAP, Holzapfel WH, Stiles ME. Enterococci at the crossroads of food safety? Int J Food Microbiol. 1999;47:1-24.

13. Lapointe JM, Higgins R, Barrette N, Milette S. Enterococcus hirae enteropathy with ascending cholangitis and pancreatitis in a kitten. Vet Pathol. 2000;37(3):282-284. doi:10.1354/vp.37-3-282.

14. Patel R, Piper KE, Rouse MS, et al. Determination of 16S rRNA Sequences of Enterococci and Application to Species Identi cation of Nonmotile. Society. 1998;36(11):3399-3407.

15. Fisher K, Phillips C. The ecology , epidemiology and virulence of Entero-coccus. Microbiology. 2009;(155):1749-1757. doi:10.1099/mic.0.026385-0.

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