Title: Antidermatophytic Effect of M Lucida Part Extracts (Ethanolic and Aqueous) on Selected Clinical Isolates
Authors: Owolabi, S.L, Azeez I.A, Boyejo A.O, Issah A.O
DOI: https://dx.doi.org/10.18535/jmscr/v7i9.30
Abstract
Dermatophytoses are the most frequent fungal infections worldwide, affecting individuals in various age groups and heading to worse quality of life and economic burden due to treatment expenditure. Hence this study was carried out to determine the effect of Morida lucida parts extract on dermatohytes.
Twenty-five gram (25g) of the plant sample was weighed into a 250ml sample bottle, 300ml of the different solvents were added, the solvents used were ethanol and water. The mixture was allowed to stand for 72 hours (3days) then filtered. The filtrates were concentrated using a rotary evaporator. Phytochemical screening was carried out on the extracts, Anti-dermatophytic effect of the Morinda lucida extracts was evaluated by the well plate agar diffusion method. A sterile cork borer was used to make wells (6mm in diameter) on the agar plates. Aliquots of 0.1ml of extract, was filled into each of the wells in the culture plates previously inoculated with the test organisms. Each well was labeled appropriately. Control experiments were also carried out where the holes were filled with 0.1ml of Fluconazole (200mg) as positive control. Minimum Inhibitory Concentration (MIC) and minimum fungicidal concentration (MFC) of Morinda lucida parts extracs were respectively carried out by micro tube dilution method on the isolates.
Phytochemical screening analysis revealed the presence of alkaloids, flavonoids, saponins, tannins, steroids,and glycoside. The result of MIC and MFC showed higher effect of aqueous bark extract on all the isolates, followed by ehanolic bark extract.
Aqueous bark extract was observed with the highest MIC and MFC effects on all the isolates.
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