Title: ORTs in an Infertile Woman – A Comparative Study
Authors: Virendra Prasad Pandey, Devatwal Kasturi Gabrulal, Deepa Masand Lokwani, Surabhi Tomar (Sharma)
Background: There exists a lacuna in the assessment of ovarian reserve in an aged infertile woman, which reflects their reproductive potential.
Objective: To compare and evaluate the role of serum FSH, LH, E2, Inhibin B and AMH with the ovarian follicle status in an infertile woman.
Material and Methods: A comparative observational and cross-sectional study was done in 120 females aged 18 to43 years fulfilling the inclusion and exclusion criteria. Venous blood (5 ml) collected from subjects for measurement of serum FSH, LH, E2, Inhibin B and AMH on day 3rdof menses. Serum FSH, LH, E2 and AMH levels were measured by chemiluminescent immunometric assay. Serum Inhibin B estimated by ELISA method. Antral Follicle count (AFC) and Ovarian volume (OV) was measured by ultrasonography. The serum values of FSH, LH, E2, Inhibin B and AMH were correlated with AFC and OV. P value <0.05 was considered significant.
Results: Our study showed positive correlation exist between the serum levels of FSH, LH, E2 (p< 0.001). Inhibin B values raised in <20yrs and >40yrs age groups. Serum AMH, AFC and OV values showed significant decrease with increasing age(p<0.001). The serum AMH and OV was positively related to AFC status while serum FSH, LH, E2 values was negatively related to the AFC status. Age was negatively related to AFC status.
Conclusion: AMH test predicts the reproductive potential of an aged infertile women (>35 yrs.) and reduce the use of Assisted Reproductive Techniques (ART).
Keywords: Count, ART-Assisted Reproductive Technique, FSH-follicle stimulating hormone, LH-luteinizing hormone, E2-Estradiol, OV- Ovarian volume. ORTs-ovarian reserve tests, AMH-Anti Mullerian Hormone, AFC-Antral Follicle.