Title: Study of expression and correlation of vascular endothelial growth factor with grading and staging of breast carcinoma

Authors: Dr Nataraju G, Dr S.A.K Adil, Dr Jayani.C, Dr Bharathi M

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i3.05

Abstract

Breast cancer is the most frequent malignancy in women all over the world, resulting in death in one forth of the cases. To date, many efforts are being performed to identify prognostic factors and their relevant therapeutic agents. Tumor induced angiogenesis is essential for tumor growth, invasiveness and evolution of breast cancer. It has been proven that vascular endothelial growth factor (VEGF) are essential for neovascularization and progression of breast cancer. The objective of this study is to study the expression of VEGF in breast carcinomas and to correlate the expression of VEGF with histological grade and stage of breast carcinoma. In this study, 30 cases of histological proven invasive breast carcinoma- no special type were  analyzed for vascular endothelial growth factor expression by immunohistochemical staining and its correlation with the grading and staging of breast carcinoma. Vascular endothelial growth factor expressed in 93.33% of the breast carcinomas.  According to this study there is a significant correlation between tumor size and VEGF expression (P<0.05) while there was no significant correlation between VEGF expression and grading and lymph node involvement of breast carcinoma. expression of VEGF can be used as a prognostic marker due to the significant correlation with the tumor size.

Keywords: Angiogenesis, breast cancer, TNM staging, Scar -Bloom-Richardson grading, VEGF.

References

  1. Arya R. C, Minj M. K, Tiwari A. K, Singh D, Pandey S, AtulManoharraoDeshkar. “Patterns of Breast Lesions in Patients Attending CIMS, Bilaspur, C. G.: A Retrospective Tertiary Hospital Based Study”. Journal of Evolution of Medical and Dental Sciences 2015; Vol. 4, Issue 78, September 28; Page: 13539-13546,
DOI: 10.14260/jemds/2015/1937
  2. Hanahan D, Folkman J. Patterns and Emerging Mechanisms of the Angiogenic Switch during Tumorigenesis. Cell. 1996;86(3):353–64.
  3. Singh Y . Tumor Angiogenesis: Clinical Implications. Nepal Journal of Neuroscience. 1:61-63, 2004
  4. Linderholm B, Tavelin B, Grankvist K, Henriksson R. Vascular endothelial growth factor is of high prognostic value in node-negative breast carcinoma. Journal of Clinical Oncology. 1998;16(9):3121–8.
  5. Maae E, Nielsen M, Steffensen KD, Jakobsen EH, Jakobsen A, Sørensen FB. Estimation of Immunohistochemical Expression of VEGF in Ductal Carcinomas of the Breast. Journal of Histochemistry & Cytochemistry. 2011;59 (8):750–60.
  6. Raica M,Mogoanta L, Cimpean A M,  Alexa A, Ioanovici S, Margaritescu C et al. Immunohistochemical expression of vascular endothelial growth factor (VEGF) in intestinal type gastric carcinoma. Romanian Journal of Morphology and Embryology 2008, 49(1):37–42
  7. Gasparini G. Prognostic Value of Vascular Endothelial Growth Factor in Breast Cancer. The Oncologist. 2000Jan;5(90001):37–44.
  8. Almumen, M. Immunohistochemical Expression of VEGF in Relation to Other Pathological Parameters of Breast Carcinoma. Journal of Cancer Therapy, 6, 811-820. http://dx.doi.org/10.4236/jct.2015.69089
  9. Liu Y, Tamimi RM, Collins LC, Schnitt SJ, Gilmore HL, Connolly JL, et al. The association between vascular endothelial growth factor expression in invasive breast cancer and survival varies with intrinsic subtypes and use of adjuvant systemic therapy: results from the Nurses’ Health Study. Breast Cancer Research and Treatment. 2011Sep;129(1):175–84
  10. Cimpean A, Raica M, Suciu C, Tatucu D, Sarb S, Mureşan A. Vascular endothelial growth factor A (VEGF A) as individual prognostic factor in invasive breast carcinoma Romanian Journal of Morphology and Embryology 2008, 49(3):303–308
  11. Srabovic N, Mujagic Z, Mujanovic-Mustedanagic J, Softic A, Muminovic Z, Rifatbegovic A, et al. Vascular Endothelial Growth Factor Receptor-1 Expression in Breast Cancer and Its Correlation to Vascular Endothelial Growth Factor A. International Journal of Breast Cancer. 2013;2013:1–6.
  12. Comşa S, Maria C A, Ceauşu R, Suciu C, Raica M. Correlations between vascular endothelial growth factor expression, microvascular density in tumor tissues and TNM staging in breast cancer. Biol. Sci., Belgrade, 64 (2), 409-417, 2012
  13. Ghasemi M, Emadian O, Naghshvar F, Bekhradnia A, Abediankenari S, Larijani L et al. Immunohistochemical Expression of Vascular Endothelial Growth Factor and Its Correlation with Tumor Grade in Breast Ductal Carcinoma Acta MedicaIranica, Vol. 49, No. 12 (2011) 776-779
  14. Ragab HM, Shaaban HM, Maksoud NAE, Radwan SM, Elaziz WA, Hafez NH. Expression of Vascular Endothelial Growth Factor Protein in Both Serum Samples and Excised Tumor Tissues of Breast Carcinoma Patients. International Journal of Cancer Research. 2016Jan;12 (3):152–61.
  15. Nakamura Y, Yasuoka H, Tsujimoto M, Yang Q, Tsukiyama A, Imabun S, et al. Clinicopathological Significance of Vascular Endothelial Growth Factor-C in Breast Carcinoma with Long-Term Follow Up. Modern Pathology. 2003;16(4):309–14.
  16. Ali EM, Sheta M, Mohsen MAE. Elevated serum and tissue VEGF associated with poor outcome in breast cancer patients. Alexandria Journal of Medicine. 2011;47(3):217–24.

Corresponding Author

Dr S.A.K. Adil

Department of Pathology, Mysore Medical College and Research Institute,

Mysuru, Karnataka, India

Phone number– 9019691259, Email: This email address is being protected from spambots. You need JavaScript enabled to view it.