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Category: Volume 05 Issue 11 November 2017
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Title: Characterization and Clinical Significance of Coagulase –Negative Staphylococci

Authors: Dr Sreekumary.P.K, Dr Shanimole.P.E, Dr Jayalekha.B

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i11.127

Abstract

Background: Coagulase-negative staphylococci are normal commensal of human skin. They are considered as non-pathogenic and rarely reported as to cause severe infections. But their important role as pathogen and the increased incidence of infections caused by them have been recognized in recent years. The present study is undertaken to characterize and determine the antibiogram of coagulase-negative staphylococci isolated from clinical specimen submitted to Department of Microbiology Government Medical College Kottayam.

Introduction: Coagulase- negative Staphylococci are indigenous microbial flora of human skin and mucous membrane. They are usually consider as contaminants when isolated from a clinical specimens. These organisms are becoming increasingly recognized as pathogenic agents in nosocomial infections. Virulence factor is not clearly established but role of factors such as bacterial polysaccharide components are involved in attachments and persistence of bacteria in foreign materials.

Aim of the study: The study was undertaken to identify the Coagulase negative staphylococci, their speciation and to determine the antibiotic resistance pattern.

Materials and Methods: A total of 63 isolates were collected from different samples and subjected to biochemical characterization and antimicrobial screening using conventional methods. The isolates were identified to species level by VITEK 2- compact.

Results: The common isolation was Staphylococcus epidermidis. Antibiotic susceptibility testing showed maximum resistance to Penicillin and 100% sensitivity to Vancomycin and Linezolid. The increased isolation of potential CoNS to cause infection and drug resistance among them leads to adopt laboratory procedures for identification and antibiotic pattern of CoNS, which will help the clinicians in treating the infection.

Keywords: Coagulase negative staphylococci, S.epidermidis, Erythromycin.

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Corresponding Author

Dr Sreekumary.P.K

Additional Professor, Department of Microbiology,

Government Medical College Kottayam