Title: Microbial Identification and Sequencing of Soil Bacteria from Indogangatic Plain

Authors: Priyanka, Chandrawati Jee

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i7.167

Abstract

Motive of the present experimentation is to carryout Microbial Identification And Sequencing of Soil Bacteria from Indogangatic Plain. Systematic and scientific investigation was conducted for isolation, identification and characterization of selected soil bacteria. Identification was made on the basis of 16S rRNA sequencing. 16S rRNA sequencing showed that out of all 9 isolates;5 Bacillus sp., 1 achromobacter sp., 1 Brevundimonas sp., 1 Pseudomonas sp. and 1 Ochrobactrum sp. were identified. The further investigation shows that the isolates from Patna region of Indogangatic plain is having inhibitory effect on both Gram positive and Gram negative Bacteria. On the basis of sequencing, phylogenetic tree of each isolates was prepared and a comparative study was made.

Keywords: Microbial identification, Indogangatic plain, 16S rRNA sequencing, Phylogenetic tree, soil bacteria.

References

1.      Adegboye, M.F. and Babalola, O.O. 2013. Phylogenetic characterization of cultur-ableantibiotic producing Bacillusfrom rhizospheric soils.Mol. Biol., S1: 001.doi: 10.4172/2168-9547.

2.      Basavaraj, K.N., Chandrashekhara, S., Shamarez, A.M., Goudanavar, P.S. and Manvi,F.V. 2010. Isolation and morphological characterization of antibiotic producing actinomycetes.Trop. J. Pharm. Res., 9(3): 231-236.

3.      Cook, A.E. and Meyers, P.R. 2003. Rapid identification of filamentousactinomycetes to the genus level using genus specific 16S rRNA generestriction fragment pattern.Int. J. Syst. Micobiol.,53: 1907-1915.

4.      Hill, G.T. 2000. Methods for assessing composition and diversity of soil microbialcommunity. Appl. Soil Microbiol.,15: 25-36.

5.      Khaliq, A., Mansoor-ul-Hasan.,Sagheer, M., FawadZafar, A., Hafiza Tahira Gul.,Asghar, M. and Yasir, M. 2013. Varietal screening and development of riceweevil, Sitophilusoryzae(L.) in advanced rice genotypes at different emperatures. Int. J. Biosci.,3(9): 287-292.

6.      Kokare, C. R., Mahadik, K.R., Kadam, S.S. and Chopade, B.A. 2004a. Isolation ofbioactive marine actinomycetes from marine sediments isolated from Goa andMaharashtra coastlines (West Coast of India). Ind. J. Mar. Sci., 33(3):248-256.

7.      Lam, K.S. 2006. Discovery of novel metabolites from marine actinomycetes. Curr.Opin. Microbiol.,9: 245-251.

8.      Maleki, H. and Mashinchian, O. 2011. Characterization of Streptomyces isolates withUV, FTIR spectroscopy and HPLC analyses. Bioimpacts, 1(1): 47-52

9.      Ravikumar, S., Inbaneson, S.J., Uthiraselvam, M., Priya, S.R., Ramu, A. andBanerjee, M.B. 2011.Diversity of endophyticactinomycetes from Karangkadu mangrove ecosystem and its antibacterial potential againstbacterial pathogens.J. Pharm. Res., 4: 294-296.

10.  Semedo, L.T.A.S., Linhares, A.A., Gomes, R.C., Manfio, G.P., Alviano, C.S.,Linhares, L.F. and Coelho, R.R.R. 2001. Isolation and characterization of actinomycetes from Brazilian tropical soils. Microbiol. Res., 155(4): 291-299.

Corresponding Author

Priyanka

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