Title: Comparision of A Rapid Immunochromatography Test with Elisa to Detect Rotavirus
Author: Archana Sharma
DOI: https://dx.doi.org/10.18535/jmscr/v5i7.04
Abstract
Introduction: Worldwide, one of the important causes of childhood hospitalization is diarrhea, with rotavirus being commonly implicated. ELISA is usually used to diagnose the rotavirus infection in microbiology laboratory. It is time consuming and requires sophisticated equipments. We have compared a rapid Immunochromatography test with ELISA in our study. Aim and objective: To detect the presence of rotavirus antigen in the stool sample using ELISA and immunochromatography assay and to know the sensitivity and specificity of Immunochromatography assay compared to ELISA test.
Materials and Methods: 120 stool samples were collected from the children admitted to the diarrhea ward of Bapuji and Chigateri hospital during June 2010 to May 2011. Stool samples were tested for the presence of rotavirus antigen using ELISA and Immunochromatography assay in the microbiology laboratory at JJM medical college.
Results: Of the 120 samples, 24 samples came positive by both ELISA and Immunochromatography assay. However, two samples, which came positive by ELISA, came negative by Immunochromatography. Sensitivity and specificity of Immunochromatography was 91.6% and 97.7% respectively.
Conclusion: Immunochromatography test had a good sensitivity and specificity compared to ELISA. Hence it can be used in laboratories with lower burden of samples. It gives quick and relatively precise results.
Keywords: Rotavirus, diarrhea, ELISA, Immunochromatography, sensitivity, specificity.
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