Title: Study of Bacterial Isolates in Puerperal Sepsis & its Antibiotic Sensitivity Pattern

Authors: Dr Kishor V. Ingole, Dr Preeti P. Shendre

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i3.49

Abstract

  2) To find out it’s antibiotic sensitivity pattern.

 3) To find out associated risk factors for puerperal sepsis.

Materials & Methods: This Cross Sectional study was carried out for a period of 2 years from December 2014 to September 2016. The clinically suspected cases of puerperal sepsis were included in this study. Endocervical swab or secretion and blood from each case were collected following standard procedure for microscopic examination and isolation of bacteria with antimicrobial susceptibility. The isolates were identified on the basis of colony morphology, from colony Gram staining and appropriate biochemical tests. The isolates were identified per standard biochemical testing and antibiotic sensitivity testing was done as per CLSI guidelines.

Observation & Result: Out of 67 women admitted in present study, majority 54 (80.59 %) were between 20 and 29 years of age. Majority of them were uniparous (50.74 %). 46 showed bacterial growth rests of the samples were sterile. Gram negative organisms were isolated in 31 cases (67.39 %) while Gram positive organisms in 15 cases (32.60 %). Klebsiella was the most frequent isolate 13 (28.26%) followed by Staph. aureus 10 (21.73%). In the present study, out of 67 cases studied, bacteremia was present in 46 (68.65%) cases. Present study shows predominance of Gram negative bacilli (67.39%) with 32.60% isolates being Gram positive cocci.

Conclusion:  In view of the changing spectrum of the causative agents of puerperal sepsis and their antibiotic sensitivity patterns from time to time and from one hospital to another, a positive blood culture and the antibiotic susceptibility testing of the isolates are the best guide in choosing the appropriate antimicrobial therapy in treating puerperal sepsis.

Keywords: Purperal sepsis, antibiotic sensitivity pattern, blood culture.

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Corresponding Author

Dr Kishor V. Ingole

Professor & Head, Department of Microbiology

Dr V. M. Govt. Medical College, Solapur