Title: Comparision of Biofilm Production among MRSA Strains Isolated from Surgical Site Infection
Authors: Karthika Priya.S, Muthulakshmi. K, Kamalraj.M
DOI: https://dx.doi.org/10.18535/jmscr/v5i3.07
Abstract
Surgical Site Infection (SSI) remains as the major reason for morbidity and mortality in patients undergoing surgery, even with the advances in the surgical techniques, better understanding of the pathogenesis of wound infections & wound healing. The management of postoperative infection is further complicated by, formation of virulence factors like biofilm along with MRSA by the organism.To study the prevalent of organism causing SSI virulence factors like biofilm & slime formation and genotypic characterization of MRSA genes using PCR. To detect the biofilm formation gene (ica A & ica D) of Staphylococcus aureus using PCR. Pus swabs and pus aspirates were collected from 62 suspected cases of SSI from patient admitted to obstetrics and gynaecology ward in MMCH&RI. The Samples were processed according to standard Clinical Laboratory Standard Institute guidelines (CLSI).The predominant isolate was Staphylococcus aureus. The antibiotic sensitivity testing was done by, Kirby Bauer disc diffusion method. The slime production was screened by modified Congo red agar method. Prevention of SSI and control measures will not only help in reducing the incidence of SSI but also can reduce the burden on the patient. So, this study was done in our hospital to find out the predominant organism and to study their virulence factor, the resistance pattern of the organism and their control measures.
Keywords: Staphylococcus aureus, biofilm, PCR.
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