Title: Variation of Leukocyte Count in Athletes Before and After Exercise
Authors: Gunti Durga Devi, V. Malleswaramma, P Ravi Kumar
DOI: http://dx.doi.org/10.18535/jmscr/v4i2.62
Aim: To evaluate the difference in leucocyte count before and after exercise in athletes. Methods & Materials: This study was conducted by selecting twenty healthy athletes within the age group of 18-24 years. They were exposed to regular running for atleast 3 years prior to this study. Procedure was explained to them. Before starting the procedure they were advised to take rest for 1 hour. As to start the procedure heart rate and blood pressure were checked and blood samples were taken to estimate the total leukocyte count and differential leukocyte count. After taking the sample the subjects were instructed to run for 20 minutes. After stopping the running again the blood samples were taken for same parameter. For estimation of total leukocyte count Turk’s fluid was used and for differential leukocyte count the smear was stained with Leishman’s stain. Results: P value of <0.001 was considered as statistically significant. Conclusion: Leukocyte counts will increase after exercise in athletes. Key words: Turk’s fluid, Leishman’s stain, Cytokines, Interleukins 1. Text book of Physiology. Prof. GK PAL, 2nd Edition 2001. 2. CHRISTER MALM, RODICA LENKEI & BERTIL SJODIN. Effects of eccentric exercise on the immune system in men. J Appl Physiol 86: 461 – 468, Feb 1989. 3. BAJZ, KANTORSKI J, MAJEWSKA E, ZEMAN K, POKOCA L, FORNALCZK E, TCHORZEWSKI H, SOLOWSKA Z, LEWICKI R. Immunological status of competitive cyclists before and after the training season, Int J Sports Med. 1994 Aug; 15 (6) : 319 – 24. 4. CHISTENSEN RD, HILL HR. Exercise induced changes in the blood concentration of leukocyte populations in teenage athletes. Am J Pediatr Haematol Oncol 1987 Summer; 9 (2) : 140 – 2. 5. MC CARTHY DA, DALE MM. The leucocytosis of exercise. A review and model. Sports Med. 1988 Dec; 6 (6) : 333 – 63. 6. DAN NEMET, MD, YOUNGMANOH, PHD, HO-SEONG KIM. MD, MARY ANN HILL, PHD & DAN M. COOPER, MD. Effects of intense exercise on inflammatory cytokines and growth mediators in adolescent boys, Paediatrics Vol. 110. No. 4. October 2002, pp 681-689. 7. ELIKIM A, WOLACH B, KODESH E, GAVRIELI R, RANDAY J, BEN-TOVIM T, YAROM Y, FALS B. Cellular and humoral immune response to exercise among gymnasts and untrained girls. Int J Sports Med. 1977 Apr; 18 (3) 208 – 12. 8. GABRIEL H, SCHWARZ L, BORN P, KINDERMANN W. Differential mobilization of leukocyte and lymphocyte subpopulations into the circulation during endurance exercise. Eur J Appl. Physiol Occup Physiol. 1992; 65 (6): 529-34. 9. STEPHAN R VAN EDDEN, JOHN GRANTON, JENNIFER M. HARDS, BARBAR A MOORE, AND JAMES C. HOGG. Expression of the cell adhesion molecules on leukocytes that de marginate during acute maximal exercise. J. Appl Physiol 86; 970 – 976, 1999. 10. BENTE KLARLUND PEDERSEN. The cellular immune system and muscular activity. The Center of Muscle Research, Department of Infectious Diseases, DenmarkAbstract
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