Title: A Study on Antibiotic Sensitivity Pattern in Biofilm Positive and Negative Isolates of Pseudomonas Aeruginosa Isolated From Clinical Samples
Authors: Sowmya Nasimuddin, Jeevan Malaiyan, Mohanakrishnan Kandaswamy, Priyadharshini Parthasarathy, Sumathi Gnanadesikan, Savitha S, Kamal Raj M
DOI: http://dx.doi.org/10.18535/jmscr/v4i3.38
Pseudomonas aeruginosa, a potential hospital pathogen with the tendency to form biofilm and multidrug resistance. Our study is to observe the correlation between biofilm formation by Pseudomonas aeruginosa and an accelerated increase in frequency of multidrug resistance when compared to non-biofilm producers. A total of 100 isolates of Pseudomonas aeruginosa [Pus (59%), urine (21%), sputum (16%) and other samples (4%)] were taken for our study. Biofilm formations by tube method were found in 86 isolates (86%) with 85% (17/21) of the urinary isolates forming biofilm. Resistance to Carbapenam, Cephalosporins and other anti – Pseudomonal antibiotics were clearly observed to be more in biofilm producing isolates than non biofilm producers. KEY WORDS: Pseudomonas aeruginosa, Biofilm, Tube method. 1. Marilyn PG, José VB, Santiago NC. Overview of Multidrug-Resistant Pseudomonas aeruginosa and Novel Therapeutic Approaches Journal of Biomaterials and Nano biotechnology 2012; 3: 519-27 2. Dardi CK, Dr.Wankhede SV A study of Biofilm formation & Metallo-β-Lactamases in Pseudomonas aeruginosa in a tertiary care rural hospital. International Journal of Scientific and Research Publications 2013;3 (10):2250-3153 3. Colle JG,Mles RB,Watt B 1996.Tests for identification of bacteria.In: Collee JG, Fraser AG, Marmon BP, Simmons A, Editors. Mackie and McCartney Practical Medical Microbiology,14 th ed. Churchill Livingstone, New York, Pp 131 – 49 4. Ryder C, Byrd M, Worzniak DJ Role of polysaccharides in Pseudomonas aeruginosa biofilm development Curr Opin Microbiol 2007;10:644-48. 5. Chaudhary M, Payasi A (2013) Rising Antimicrobial Resistance of Pseudomonas aeruginosa Isolated from Clinical Specimens in India. J Proteomics Bioinform 6:005-009. doi:10.4172/jpb.1000184 6. Sachinkumar W, Vivek I , Ghadge D.P, Bhore A.P Hospital based infections of Gram negative organisms: Study Report. Indian Journal of Basic & Applied Medical Research; June 2013: Issue-7, Vol.-2, P. 797-800. 7. Alicia VZ, Francyelle CM, Monique SC, Adriana de MM, Cristina AM, Thiago FF, Valério MN, Patrícia de MSF. Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of Pseudomonas aeruginosa Strains Isolated from Clinical Samples. Journal of Biomaterials and Nanobiotechnology, 2013, 4, 98-106. 8. CLSI (Clinical and Laboratory Standards Institute). (2007). Performance standards for antimicrobial disk susceptibility tests, 9th edn. Approved Standard M2 , A9.Wayne,PA: document M100 - S17. 9. Koneman EW, Allen SD, Janda WM, Schreckenbergr PC, Winn WC. Antimicrobial susceptibility testing, chapter 15. In: color Atlas and Textbook of Diagnostic Microbiology, 5th edition ( Lippicott, Philadelphia ) 1997 ;785. 10. G. Lucchetti, A. J. Silva, S. M. Y. Ueda, M. C. D. Perez and L. M. J. Mimica, “Infecções Do Trato Urinário: Análise da Freqüência e Do Perfil de Sensibilidade Dos Agentes Causadores de Infecções Do Trato Urinário Em Pacientes Com Cateterização Vesical Crônica,” Journal Brasileiro de Patologia e Medicina Laboratorial , Vol. 41, No. 6, 2005, pp. 383-389. 11. Carlos J Sanchez Jr, Katrin Mende, Miriam L Beckius, Kevin S Akers, Desiree R Romano,Joseph C Wenke and Clinton K Murray, Biofilm formation by clinical isolates and the implications in chronic infections BMCInfectious Diseases 2013, 13:47 12. Javiya VA, Ghatak SB, Patel KR, Patel JA. Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India. Indian J Pharmacol 2008;40:230-4 13. Niels Hoiby, Thomas Bjarnsholt, Michael Givskov, Soren Molin, Oana Ciofu. Antibiotic resistance of bacterial biofilms. International Journal of Antimicrobial Agents 35 (2010) 322–32.Abstract
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