Title: Induced To Differentiation and Morphogenesis of Pancreatic Islet Clusters: Effects on Glycated Fetal Bovine Serum
Authors: Sivalingam Murugan, Ikuo Nishigaki, Gowri Rangasamy Gunassekaran, Ganapathy Ekambaram, Kalpana Deepa Priya Dorayappan, Vishal Babu, Ameesh Madhusoodhanan Nair
DOI: http://dx.doi.org/10.18535/jmscr/v4i5.17
Mouse embryonic stem cells (MESCs) are potency to differentiate into mature pancreatic islet cells and using for tissue repair treatment. Advanced glycation end-products (AGEs) are accrual of long lived proteins of various tissues and have been implicated in chronic complications in diabetes mellitus, but the pathogenic role of AGEs on pancreatic islet cells is remains unclear. Stem cell differentiation is carried out based on standard protocol followed in the laboratory. In results the differentiated stem cells shows extensive self-renewal capacity and grow in highly viable for long term in the culture media, had remarkable size, formed aggregates, which built tissue like structures and closer examination by light microscopy analysis revealed islet cell like morphology. Administration of GFBS +iron on induced pancreatic islet cells is observed induce charity of cells. Conclusion this findings demonstrate that AGEs directly cause insulin secretory defects and toxicity, most likely by impairing pancreatic cell functions, which may contribute to the development of diabetes. This approach offers a promising an ample supply of insulin producing cells for cell transplantation therapy and maintains the complication factors in diabetic mellitus, suggesting that further improvement is needed. Keywords: Embryonic stem cell, insulin producing cell, diabetes, Glycated bovine albumin 1. International Diabetes Federation (IDF). IDF Diabetes Atlas Update Poster, 6th ed.; International Diabetes Federation: Brussels, Belgium, 2014. 2. Morrish, N. Wang, S.L. Stevens, L. Fuller, J. Keen, H. Mortality and causes of death in the who multinational study of vascular disease in diabetes. Diabetologia, 2001, 44: 14-21. 3. Johnson, B.V.N. Shindo, P.D. Rathjen, J. Rathjen, J. Keough R.A. Understanding pluripotency- how embryonic stem cells keep their options open. 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