Title: Development of a novel Computer Assisted system for human Sperm Head Morphometry based on open source software

Authors: Raghavendra Maggavi, Sanjay Pujari, Vijay Kumar C.N

 DOI:  http://dx.doi.org/10.18535/jmscr/v4i6.63

Abstract

Computer assisted sperm head morphology systems have been commercially available since the mid-1980s. The goal of these systems has been to obtain objective data on sperm head that can be used in research, human fertility clinics, and animal breeding programs. Widely used commercial systems include the Hamilton-Thorne, Bioscience, Beverly, Massachusetts, The commercial systems differ in their grayscale bit-depth, search region for finding the sperm in the image, grayscale thresholding method, image segmentation method to determine the pixel coordinates of the sperm head, A large amount of  information necessary to reproduce data obtained with commercial systems is proprietary information not easily available, eventually limits the ability to conduct comparative evaluation and also its elevated cost has slowed down widespread adoption. In the present study, we have developed a plugin for sperm head morphology using open source software. Described the systems functionality and confirmed its validity with respect to the commercial softwares.

Keywords- Microscopic image, Spermatozoon, IUI, IVF, ICSI, WHO, IMAGEJ, Medea LAB CASA 

References

    

1.      Raghavendra. Maggavi, Sanjay. Pujari, Amar. Herekar. “Analysis of human sperm head morphology using open- source software”. International Journal of Engineering Science and Computing.Vol.6, pp.5597-5602, 2016

2.      Raghavendra. Maggavi, Sanjay. Pujari, Vijay Kumar C.N. “Assessment of Human Sperm Head Morphology for Assisted Reproduction Techniques using Open Source Software”. International Journal of Advanced Research in Electrical, Electronics and Instrumentation Engineering. Vol. 5, pp.4618-4626, 2016.

3.      Check JH(1), Adelson HG, Schubert BR, Bollendorf A. “Evaluation of sperm morphology using Kruger's strict criteria”. International Journal of Archive Andrology. Vol; 28, pp.15-7. 1992

4.      Eustache F, Auger J. “Inter-individual variability in the morphological assessment of human sperm: effect of the level of experience and the use of standard methods”. International Journal of Human Reproduction; Vol.18, pp. 1018-22. 2003

5.      Enginsu, M.E., Pieters, M.H.E.C., Dumoulin, J.C.M. et al. “Male factor as determinant of in-vitro fertilization outcome”. International Journal of Human Reproduction, Vol.7, pp.1136-1140. (1992a)

6.      Enginsu, M.E., Dumoulin, J.C.M., Pieters, M.H.E.C. et al. “Comparison between the hypoosmotic swelling test and morphology evaluation using strict criteria in predicting in vitro fertilization (IVF)”. Journal of Assisted  Reproducrion. Genet, Vol.9,pp. 259-264. (1992b)

7.      Coetzee, K., Kruger, T.F., Menkveld, R. et al. “Usefulness of sperm penetration assay in fertility predictions”.International journal of Archive Andrology., Vol.23, pp.207-212. 89

8.      Franken, D.R., Oehninger, S., Burkman, L. et al. “The hemizona assay (HZA): a predi-ctor of human sperm fertilizing potential in in vitro fertilization (IVF) treatment”. International journal of Invitro Fertility. Embryo Transfer, Vol.6, pp.44-49. 1989

9.      Chan, S.Y.W., Wang, C., Chan, S.T.H. et al. “Differential evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human oocytes”. International Journal of  Human Reproduction,Vol.5, pp. 84-88. 1990

10.  Henkel, R., Muller, C., Miska, W. et al. “Determination of the acrosome reaction in human spermatozoa is predictive of fertilizationin vitro”. Int. Journal of Human Reproduction,Vol.8, pp.2128-2132. 1993

11.  Kruger, T.F., Menkveld, R., Stander, F.S.H. et al. “Sperm morphologic features as a prognostic factor in in vitro fertilization”. International Journal of Fertility. Sterility., Vol.46, pp.1118-1123. 1986

12.  Grow, D.R., Oehninger, S., Seltman, H.J. et al. “Sperm morphology as diagnosed by strict criteria: probing the impact of teratozoospermia on fertilization  rate  and  pregnancy  outcome  in  a  large  in  vitro fertilization population”. International Journal of Fertility Sterility., Vol.62, pp.559-565. 1994

13.  Kruger, T.F., Acosta, A.A., Simmons, K.F. et al. “Predictive value of abnormal sperm morphology in vitro fertilization”. International Journal of Fertility Sterility., Vol.49,pp.112-117. 1988

14.  Coetzee, K., Kruger, T.F. and Lombard, C.J. “Predictive value of normal sperm morphology: a structured literature review”. International Jou. of Human Reproduction. Update, Vol.4, pp.73-82. 1998

15.  Figueiredo, H., Tavares, A., Ferras, L. et al. “Isolated teratozoospermia and in vitro fertilization”. Inter. Journal of Assisted Reproduction. Genet.,13, 64-68. 1996

16.  Oehninger, S., Acosta, A.A., Morshedi, M. et al. “Corrective measures and pregnancy outcome in in vitro fertilization in patients with  severe  sperm  morphology  abnormalities”.  International Journal of Fertility Sterility., Vo.l50, pp.283-287. 1988

17.  Yue, Z., Meng, F.J., Jorgensen, N. et al. “Sperm morphology using strict criteria after Percoll density separation: influence on cleavage and pregnancy rates after in-vitro fertilization”. International Journal of Human  Reproduction., Vol.10, pp.1781-1785. 1995

18.  Eliasson,  R.  “Standards  for  investigation  of  human  semen? Andrologia”, Vol.3,pp. 49-64. 1971 

19.  World Health Organization  WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, 1st edn. Press Concern, Singapore. 1980

20.  Mahadevan, M.M. and Trouson, A.O. “The influence of seminal characteristics on the success rate of human in vitro fertilization”. International Journal of Fertility Sterility., Vol.42, pp.400-405. 1984 

21.  Liu, D.Y. and Baker, H.W.G. “Relationship between human sperm acrosin,acrosomes, morphology  and  fertilization  in  vitro”.  International Journal of Human Reproduction., Vol.5, pp.298-303. 1990

22.  Menkveld, R., Stander, F.S.H., Kotze, T.J.vW. et al. “The evaluation of morphological characteristics of human spermatozoa according to stricter criteria”. International Journal of Human Reproduction, Vol.5, pp.586-592. 1990

23.  Ombelet, W., Fourie, F.leR., Vandeput, H. et al. “Teratozoospermia and in-vitro fertilization: a randomized prospective study”. International Journal of Human Reproduction., Vol.9, pp.1479-1484. 1994

24.  Hernandez, M., Molina, R., Olmedo, J. et al. “Prognostic value of the strict criteria: an Argentinian experience”. International Jou. of Archive Andrology.Vol.37,pp.85-87. 96

25.  World Health Organization  WHO Laboratory Manual for the Examination   of   Human   Semen   and   Semen-Cervical Mucus Interaction, 2nd edn. Cambridge University Press, Cambridge. 1987

26.  World Health Organization  WHO Laboratory Manual for the Examination   of   Human   Semen   and   Semen-Cervical Mucus Interaction, 3rd edn. Cambridge University Press, Cambridge. 1992

27.  Hernandez, M., Molina, R., Olmedo, J. et al. “Prognostic value of the strict criteria: an Argentinian experience”. International Journal of Archive Andrology., Vol.37, pp.85-87. 1996

28.  Kruger, T.F. “Computer-assisted sperm analysis systems: Morphometric aspects”. International Journal of  Human Reproduction. Vol.10, pp.46-52. 1995  

29.  Davis, R.P., Bain, D.E., Siemers, R.J. et al. “Accuracy and precision of the Cell Form-Human automated sperm morphometry instrument. International Journal of Fertility Sterility. Vol.58, pp.763-769. 1992

30.  Wang,C, Leung, A., Tsoi, W.-L. et al. “Computer-assisted assessment of human sperm morphology: usefulness in predicting fertilizing capacity of human  spermatozoa”.  International Journal of  Fertility Sterility. Vol.55, pp.989-993. 1991a

31.  Wang, C., Leung, A., Tsoi, W.-L. et al. “Computer-assisted assessment of human sperm morphology: comparison with visual assessment”. International Journal of  Fertility Sterility. Vol.55, pp.983-988. 1991b

32.  Kruger, T.F., Du Toit, T.C., Franken, D.R. et al. “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading”. International Journal of  Fertility Sterility. Vol.59, pp.202-209. 1993

33.  Garrett, C. and Baker, H.W.G. “A new fully automated system for the Morphometric analysis of human sperm heads”. International Journal of Fertility Sterility. Vol.63, pp.1306-1317. 1995

34.  Menkveld, R. and Kruger, T.F. “Advantages of strict (Tygerberg) criteria for evaluation of sperm morphology”. International Journal of Andrology., Suppl. Vol.2, pp.36-42. 1995

35.  Donnelly, E.T., Lewis, S.E., McNally, J.A. and Thompson, W. “Invitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome”. International Journal of Fertility Sterility. Vol.70, pp.305-314. 1998

36.  Hammadeh, M.E., Stieber, M., Haidl, G. and Schmidt, W. “Association between sperm cell chromatin condensation, morphology based on strict criteria, and fertilization, cleavage and pregnancy rates in an IVF program”. Andrologia, Vol.30, pp.29-35. 1998

37.  Menkveld, R. and Kruger, T.F. “Advantages of strict (Tygerberg) criteria for evaluation of sperm morphology”. International Journal of Andrology., Suppl. Vol.2, pp.36-42. 1995

38.  Lacquet, F.A., Kruger, T.F., Du Toit, T.C. et al. “Slide preparation and staining procedures”. 1996

39.  Davis, R.O. and Gravance, C.G. “Standardization of specimen preparation, staining, and sampling methods improves automated sperm-head morphometry analysis”. International Journal of Fertility Sterility. Vol.59, pp.412-417. 1993

40.  WHO WHO Laboratory Manual  for the Examination  of Human Semen and Sperm-cervical Mucus  Interaction,  4th edn. Cambridge Uni. Press, Cambridge,UK. 1999

41.  Chan, S.Y.W., Wang, C., Chan, S.T.H. et al. “Predictive value of sperm morphology and movement characteristics in the outcome of in vitro fertilization of human oocytes”. International Journal of Invitro Fertility. Embryo Transfer, Vol.6, pp.142-48. 1989

42.  Cooper TG, Neuwinger J, Bahrs S, Nieschlag E.   “Internal quality control of semen analysis. International Journal of Fertility Sterility;Vol.58: pp.172-8, 1992

43.  Cooper TG, Atkinson AD, Nieschlag E. “Experience with external quality control in spermatology”. International Journal of Human Reproduction; Vol.14: pp.765-9. 1999

44.  WHO (1999) WHO Laboratory Manual for the Examination of HumanSemen and Sperm-cervical Mucus Interaction, 4th edn. Cambridge University Press, Cambridge, UK

Corresponding Author

Raghavendra Maggavi

Research Scholar

Visvesvaraya Technological University, Belagavi, India