Title: Screening of milk samples for sub-clinical and clinical mastitis by using CMT and SCC
Authors: R. Lakshmi, K.K. Jayavardhanan
DOI: http://dx.doi.org/10.18535/jmscr/v4i6.27
The present study was conducted to screen the sub-clinical and clinical mastisis samples collected from Kerala veterinary University Livestock Farm, Mannuthy, by using California mastitis Test and Somatic cell count. A total of 80 lactating crossbred cows were subjected to following screening test for detecting sub-clinical mastitis (SCM) and clinical mastitis. Screening results revealed 28 cows were positive for sub-clinical mastitis (35 per cent), 19 cows were positive for clinical mastitis (23.75 per cent) and 33 cows were found to healthy (41.25 per cent). These three groups of samples were subjected for somatic cell count. A significant (P<0.01) increase in milk somatic cell count SCC in subclinical and clinical mastitis affected cows were noticed when compared with healthy crossbred cows. SCC is was found to be the most accurate method for detecting subclinical mastitis. Key Word: Mastisis, California mastitis Test and Somatic cell count. 1. Dhumka, V.K and Srivastava, A.K. 2003. Pharmacology of antibiotics used in the treatment of mastitis: An overview. Proceedings on 4th round table on mastits, IVRI, Izatnagar. 97-111 2. Mellenberger, R. 2001. California Mastitis Test (CMT): An Invaluable Tool forManaging Mastitis, Dept. of Animal Sciences, Michigan State University. 2001:1. 3. Miller, K., Brezinska-Slebodzinska, E., Madsen F. C. 1993. Oxidative stress, antioxidants and animal function. J. Dairy Sci. 76: 2812-2823. 4. Oliveira, A.P., Watts, J.L., Salmon, S.A and Aarestrup, F.M. 2000. Antimicrobial susceptibility of S.aureus isolated from bovine mastitis and the United States, J. Dairy Sci., 83: 855-862. 5. Packard, V.S., Tatini, S., Fugua, R., Heady, J and Gilman, C. 1992. Direct microscopic methods for bacteria or somatic cells, In Methods for the Examination of Dairy Products, 309–321 6. Peeler, E. J., Green, M. J., Fitzpatrick, J. L., Morgan, K.L., Green, L.E. 2000. Risk factors associated with clinical mastitis in low somatic cell count British dairy herds. J. Dairy Sci., 83: 2464-2472. 7. Saluja, P.S., Gupta, S.L., Kapur and Sharma, A. 2005. Antibiogram of bacterial isolates of bovine intramammary origin. Indian vet. J., 82: 323-324. 8. Schukken, Y.H., Mallard, B.A., Dekkers, J.C., Leslie, K.E, Stear, M.J. 1994. Genetic impact on the risk of intramammary infection following Staphylococcus aureus challenge. J. Dairy Sci., 77: 639-647. 9. Sharma, A and Prasad, B. 2003. Prevalence and therapy of mastitis in dairy animals of Kangra valley of Himachal Pradesh., Proceedings on 4th round table on mastitis . 10. Sharma, N., Maiti, S.K and Sharma, K.K. 2007. Prevalence, Etiology and Antibiogram of Micro-organism associated with sub-clinical mastitis in buffaloes in Durg, Chhattisgarh state (India). Int. J. Dairy Sci., 1: 7-12. 11. Shuster, D.E., Lee, E.K and Kehrli, M.E. 1996. Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation. Am. J. Vet. Res., 57: 1569-1575. 12. Sordillo, L.M., Shafer-Weaver, K. and DeRosa, D. 1997. Immunobiology of the mammary gland. Journal of Dairy Science, 80: 1851-1865.Abstract
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