Title: Comparative Study of Rapid Diagnostic Test Versus Elisa For HIV Detection At Blood Bank In Tertiary Care Hospital

Authors: Dr Parth R.Goswami, Dr Jitendra J. Chavda

 DOI:  http://dx.doi.org/10.18535/jmscr/v4i8.29

Abstract

BACKGROUND: Human immunodeficiency virus leads to development of AIDS which is one of the major public health problem worldwide.1,2 So it is necessary to diagnose HIV as early as possible for prevention of HIV spread in community

AIM: This study help us to evaluate effectiveness of rapid diagnostic kits as compared to ELISA methods.

MATERIALS AND METHODS: This study was carried out at tertiary care hospitals (blood bank),Gujarat , India from the date 01/01/2016 to 31/07/2016.We have tested total 9111 donor samples with using fourth generation ELISA kit as well as Rapid diagnostic kit.

RESULTS:  Rapid diagnostic tests having Sensitivity of 90.90%. specificity was 100%,positive predictive value 100% and negative predictive value 99.98%. and accuracy was  99.98% .

CONCLUSION: The rapid diagnostic HIV kits assay gives result in 15 minute can be used in emergency situations when there is less time available to perform ELISA test. So as sensitivity and specitivity of rapid diagnostic tests are high it can be used in emergency situation whenever needed

KEYWORDS: ELISA, Human immunodeficiency virus, p24 antigen, Rapid diagnostic test. 

References

 

    1.      United States Centers for Disease control and Prevention. HIV Surveillance - United States, 1981-2008. 2012.

2.      WHO Report. Global Summary of the AIDS epidemic. World Health Organization 2009 Available from: http://www.who.int/-hiv/data/2009_global_summary.png 

3.      Cohen T, Corbett EL. Test and treat in HIV: Success could depend on rapid detection. Lancet 2011;378:204-6.

4.      Metcalf JA, Davey RT, Lane HC. Acquired immunodefi- ciency syndrome: serologic and virologic tests. In: Devita VT, Hellman S, Rosenberg SA, eds. AIDS: biology, diagnosis, treatment and prevention, 4th edn. Philadelphia: Lippincott Raven, 1997; 177–195

5.      Zhou H, Jiang CW, Li SJ, Li H, Huang MQ, Liang JQ, et al. Evaluation of four kits for screening HIV antibody. Zhonghua Yu Fang Yi Xue Za Zhi 2010;44:247-50. 

6.      Brust S, Duttmann H, Feldner J et al. Shortening of the diagnostic window with the new combined HIV p24 antigen and anti-HIV-1 ⁄ 2 ⁄ O screening test. J Virol Methods 2000; 90: 153–165.

7.      Laperche S, Maniez-Montreuil M, Courouce´ AM. Screening tests combined with p24 antigen and anti-HIV antibodies in early detection of HIV-1. Transfus Clin Biol 2000; 7(suppl 1): S18–S24.

8.      Gu¨rtler L, Mu¨hlbacher A, Michl U et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods 1998; 75: 27–38.

9.      Ly TD, Laperche S, Courouce´ AM. Early detection of human immunodeficiency virus infection using third- and fourth-generation screening assays. Eur J Clin Microbiol Infect Dis 2001; 20: 104–110.

10.  Weber B, Fall EMB, Berger A, Doerr HW. Reduction of diagnostic window by new fourth-generation human immunodeficiency virus screening assays. J Clin Microbiol 1998; 36: 2235–2239

11.  Ly TD, Martin L, Daghfal M et al. Seven human immunodeficiency virus (HIV) antigen–antibody combination assays: evaluation of HIV seroconversion sensitivity and subtype detection. J Clin Microbiol 2001; 39: 3122–3128

12.  Saville RD, Constantine NT, Cleghorn FR et al. Fourthgeneration enzyme-linked immunosorbent assay for the simultaneous detection of human immunodeficiency virus antigen and antibody. J Clin Microbiol 2001; 39: 2518–2524.

13.  Van Binsbergen J, Siebelink A, Jacobs A et al. Improved performance in seroconversion with a 4th generation HIV antigen ⁄ antibody assay. J Virol Methods 1999; 82: 77–84.

Corresponding Author

Dr Parth R.Goswami

“102” Sangath flat, Shilpinagar, Kalanala, Bhavnagar-364001

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