Title: Silver Stained Nucleolar Organising Region (AgNOR) Count and its Correlation with Colposcopy in Suspicious Cervical Pathology
Authors: A.R.Nasreen Fathima, S.Thangathai
DOI: http://dx.doi.org/10.18535/jmscr/v4i8.68
Introduction: Colposcopy is considered a sensitive tool for screening of cervical malignancy. Colposcopy directed biopsy helps in confirming diagnosis. Silver stained nucleolar organising region (AgNOR count) of dysplastic cells further enhances the diagnosis. Aim: To study the correlation of AgNOR count and colposcopy in suspicious cervical lesions. Materials and methods: 200 women of reproductive age group were screened for cervical cancer and colposcopy directed biopsy taken. AgNOR count studied and correlated with HPE and Modified Reid’scolposcopic index. Results: There was a good correlation between AgNOR count and the degree of dysplasia. Also there was a good correlation of AgNOR count and Modified Reid’scolposcopic index. Conclusions: AgNOR count is simple, effective method and it adds strength to histopathology and colposcopy and also assess different grades of cervical intraepithelial neoplasia. Keywords: colposcopy, cervical intraepithelial neoplasia, AgNOR count, Reid’s colposcopic index. 1. J Fam Pract. 1994 Jul;39(1):65-70. Reid's Colposcopic Index. Ferris DG(1), Greenberg MD. 2. Rowlands DC. Nucleolar organizer regions in cervical intraepithelial neoplasia. J Clin Pathol. 1988; 41:1200–1202. doi: 10.1136/jcp.41.11.1200 3. Platon D, Menanger M, Jeannesson P, et al. Improvement of staining in visualization of agyrophillic proteins of the nucleolar organizing region at optical level. Histochem J. 1986;18:5–14. doi: 10.1007/BF01676192 4. Underwood JCE, Giri DD. Nucleolar organizing regions as diagnostic deterimants for malignancy (editorial) JPathol. 1988; 155:95–96 .doi:10.1002/path.1711550203. 5. Kuppuswamy B. Manual of Socioeconomic Status (Urban) 1st ed. Delhi: Manasayan; 1981. pp. 66–72 6. Stewart L, Collins YC. Strength of correlations between colposcopic impression and biopsy histology. Gynecol Oncol. 2003; 89:424–428. doi: 10.1016/S0090-8258(03)00082-9. 7. Lozowski MS, Mishriki Y, Talebian F, Solitare G. The combined use of cytology and colposcopy in enhancing diagnostic accuracy in preclinical lesions of the uterine cervix. Acta Cytol. 1982;26:285–291. [PubMed] 8. Samarth Shukla et al., Study of Nucleolar Organizer Regions – as Tumor Proliferative Marker in Cervical Smears. Journal of Clinical and Diagnostic Research. 2013 February, Vol-7(2): 278-283282 282 9. Misra JS, Das V, Srivastava AN, Singh U, Singh M. AgNOR counts in cervical smears under normal and other cytopathologic conditions. Anal Quant CytolHistol 2005;27:337-40 10. Singh U, Singh R, Srivastava An, et al. AgNOR count and its diagnostic signific-ance in cervical intraepithelial neoplasia. ObstetGynecol India. 2006;56 (3):244–6 11. Egan MJ, Freeth M, Croker J. Relationship between intraepithelial neoplasia of the cervix and the size and number of the nucleolar organizer regions. GynaecolOncol. 1990;36:147–151. doi: 10.1016/0090-8258(90)90104-S. 67. 12. Pratibha D, Kuruvilla S. Value of AgNORs in premalignant and malignant lesions of cervix. Indian J PatholMicrobiol. 1995; 38 (1):11–16 13. Kaushik R, Sharma V, Gulati A, et al. AgNOR counts in cervical lesions. Indian J Pathol Microbiol. 2003;46(2):201–203. [PubMed] 14. J Obstet Gynaecol India. 2012 Aug; 62(4): 437–441. doi: 10.1007/s13224-012-03003 PMCID: PMC3500951Original Article, AgNOR Count and its Correlation with Colposcopy in Suspicious Cervix, Ritu Goyal, 1,3 Kaur Manjit Mohi,1 and Manjit Bal Singh.Abstract
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