Title: The Effect of Different Techniques of Microwave Disinfectionon Elastomeric Impression Materials (Laboratory and Microbiological Study)
Authors: Ahmed Abd El-Hamid, Enaya Shararah, Dawlat Mostafa, Shams Arafa, Eman Sabry
DOI: https://dx.doi.org/10.18535/jmscr/v4i10.57
Dental impressions are often contaminated by exposure to saliva and blood. Microwave irradiation and immersion in solutions have been recommended for denture disinfection. However, the effect on impression materials has not been studied enough. Accordingly, the question is, is it possible to apply "Microwave disinfection" to impression materials vinyl siloxanether, polyvinyl siloxane and polyether? And if yes is it better to use with or without hydrogen peroxide (H2O2)? The Aim of this study is to evaluate and compare the effect of different techniques of microwave disinfection (microwave for 1 minute, 3 minutes and microwave with H2O2 for 1 minute and 3 minutes) on elastomeric impression materials (laboratory and microbiological study). Martials and methods: Specimens were made with vinyl siloxanether (Group I), polyvinyl siloxane (Group II) and polyether (Group III). Experimental groups were divided into Subgroup I :No disinfection measures (control group) , Subgroup II: The disinfection of samples were carried using microwave 630 w immersed in saline for 1 minute, Subgroup III: The disinfection of samples were carried using microwave 630 w immersed in saline for 3 minutes, Subgroup IV: The disinfection of samples were carried using microwave 630 w immersed in hydrogen peroxide for 1 minute, Subgroup V:The disinfection of samples were carried using microwave 630 w immersed in hydrogen peroxide for 3 minutes. The disinfection effect on bacteria and physical properties of impressions were evaluated. Result; Different techniques were significantly different (P< .05) when compared with the control in reducing the number of colony forming units (CFU) per unit volume (mL) for both streptococcus mutans and candida albicans. The most significant reduction in the CFU/mL was noted when the disinfection of samples was carried using microwave 630 w with hydrogen peroxide for 3minutes. No statistically significant difference was noted between the control and treatment groups in terms of all the physical properties tested (P>.05). Conclusion; Microwave disinfection decreased the CFU/ml of microorganisms in dental impressions, but it is more effective when samples immersed in hydrogen peroxide. Microwave disinfection with or without hydrogen peroxide had no effect on physical properties of polyvinyl siloxanether and polyvinyl siloxane but cause dimensional changes for polyether. Keywords: microwave disinfection, elastomeric impression materials, hydrogen peroxide. 1. 1. Emphasis PS. Infection control in dental office: a realistic approach. . J Am Dent Assoc,112 ,pp. 458-68, 1986. 2. Egusa H, Watamoto T, Abe K, Kobayashi M, Kaneda Y, Ashida S, et al. 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