Title: Comparison of Rapid Fungal Staining Technique 1% Chicago Blue 6B stain for the Diagnosis of Superficial Fungal Infections (Pityriasis Versicolor/ Tinea Versicolor) with 10% KOH Mount and Routine Fungal Culture on Sabouraud's Dextrose Agar with Olive Oil Overlay
Authors: N.Kamath, R.Swaminathan, Jyoti Sonawane, Nadeem Amin
DOI: https://dx.doi.org/10.18535/jmscr/v4i11.12
Pityriasis versicolor is a common skin infection all over the world caused by Malassezia furfur. 10% KOH mount is done for all clinically suspected Tinea versicolor by taking skin scrapings. 10% KOH has shown 66.66 % positives in this study. When we add 1% Chicago Blue 6B stain it enhances the results to 93.33 % which is as good as the Gold standard that is culture on SDA with a overlay of sterile Olive Oil. Culture has shown 96.66% positivity as Malassezia furfur grows well when there is oil overlay. So 1% Chicago Blue 6B stain can be routinely incorporated in Mycology laboratories and also in Skin clinics as the procedure is simple, rapid, reliable and can be identified with a good contrast and specificity/sesitivity is as good as culture. 1% Chicago Blue 6B is more efficacious than usual 10% KOH mount in diagnosing superficial fungal infections caused by Malassezia furfur. KeyWords: 1% Chicago Blue stain(CSB), Malassezia furfur, 10% KOH, SDA with Chloramphenicol and cycloheximde. 1. S.Fonseka, C.Lim, U.Bandara & Manel D, New Contrast stain for the rapid diagnosis of Dermatophytosis and Pityriasis versicolor, Lab Medicine, Vol.42, Number 11, November 2011.pg 649-452 2. Tambosis E,Lim C, A comparison of the contrast stains ,Chicago Blue, Chlorazole black and Parker ink for the rapid diagnosis of skin and nail infections., Int J Dermatol, Aug 2012, 51(8); pg 935-938 3. Nitkita Lodha , Shital Amin Poojary, A novel contrast stain for the rapid diagnosis of Pityriasis versicolor : A comparison of Chicago sky Blue 6B stain, potassium hydroxide mount and culture. Ind Journal of Derma, vol 60,Iss. 4, 2015 pg 340-344. 4. Zhong Liu, Ping Sheng et al, Comparison of modified Chicago sky blue stain and potassium hydroxide mount for the diagnosis of dermatomycoses and onychomycoses., Journal of Microbial methods ,Vol.112,May 2105,pg 21-23 5. Lim CSH,Lim SL. New contrast stain for the rapid diagnosis of dermatophytic and candidal dermatomycosis. Arch Dermatol. 2008 Sep; 144(9); pg 1228-1229Abstract
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