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Category: Volume 09 Issue 01 January 2021
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Title: Comparative evaluation of methods for detection of Biofilm formation in urinary catheter tips and their antibiogram

Authors: Dr N.R.R. Gayathree, Dr T. Parvathi*, Dr P.Appa Rao

 DOI: https://dx.doi.org/10.18535/jmscr/v9i1.06

Abstract

Background: A biofilm is an accumulation of microorganisms embedded in an exopolysaccharide matrix of microbial host origin called polysaccharide intracellular adhesion (PIA). They pose a significant threat to indwelling devices, causing slow persistent infections, thereby interfering with antimicrobial therapy.

Objectives: This study was conducted to isolate and compare three conventional methods for the detection of Biofilm formation and their antimicrobial susceptibility pattern.

Materials and Methods: A total of 120 urinary catheter tips were collected from medical and dialysis units from May 2019 to November 2019. Biofilm production was detected by Congred agar, Tube method, Tissue culture methods. Antibiotic susceptibility test of Biofilm producing organisms was performed according to CLSI guidelines.

Results: Out of 120 samples 52 (43.33%), showed culture positivity, out of which bacteria are 37(71.2%) which includes E.coli 15(28%), klebsiella 10(19%), pseudomonas 6(11.5%), proteus 4(7%), Enterococci 2 (3.8%), and fungal culture revealed Candida 15 (28.8%). Out of 52 culture positives, 23(44.23%) produced Biofilm. The rate of detection by CRA, TM TCP methods was 23%, 36%, 44%, respectively. Higher antibiotic resistance was observed in Biofilm producing bacteria.

Conclusion: The TCP method of biofilm detection is quantitative and more reliable than TM and CRA methods; it can be recommended as a general screening method for the detection of Biofilm producing bacteria in laboratories.

Keywords: Biofilm of urinary catheter tips, Nosocomial infections, TCP, TM, CRA methods.

References

  1. Sharma, Divakar & Misba, Lama & Khan, Asad. (, 2019). Antibiotics versus Biofilm: An emerging battleground in microbial communities. Antimicrobial Resistance & Infection Control. 8. 10.1186/s13756-019-0533-3.
  2. Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods for biofilm formation in the clinical isolates. Braz J Infect Dis 2011;15: 305-1
  3. Johannes K. M. Knobloch, Matthias A. Horstkotte, Holger Rohde, Dietrich Mack Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. Medical Microbiology and Immunology October 2002, Volume 191, Issue 2, pp 101-106.
  4. Samant Sharvari A and Pai Chitra G. Evaluation of different detection methods of biofilm formation in clinical isolates of Staphylococci Int J Pharm Bio Sci 2012 Oct; 3(4): (b) 724 – 733.
  5. Bailey and Scott’s Diagnostic Microbiology, 13th edition by Patricia M. Tille. Elsevier publications, 2014: 35.
  6. Donlan RM, Costerton W. Biofilms: Survival mechanisms of clinically relevant Microorganisms. Clin Microbiol Rev 2002;15(2):167-93
  7. Thomas D, Day F. Biofilm formation by plant-associated bacteria. Ann Rev Microbiol 2007; 61:401-22.
  8. Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358(9276):135-8.
  9. Rise of Antimicrobial Resistance in ICU. The Critical Opinion July- Sep 2015:Issue 6.
  10. Yasmeen Taj, Farhan Essa, Faisal Aziz, Shahana U. Kazmi .Study on Biofilm forming properties of clinical isolates of Staphylococcus aureus. J Infect DevCtries2012; 5(6):403-409
  11. C. Maldonado, C. Silva de Ruiz, M.Cecilia and M.E. Nader-Macias. A simple technique to detect Klebsiella biofilm forming-strains. Inhibitory potential of Lactobacillus fermentum CRL 1058 whole cells and products. Communicating Current Research and Educational Topics and Trends in Applied Microbiology 2007: 52-59.
  12. Christensen GD, Simpson WA, Younger JA et al. Adherence of coagulase-negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol 1995;22:996-1006.
  13. R. Rewatkar, Dr. B. J. Wadher Staphylococcus aureus and Pseudomonas aeruginosa Biofilm formation Methods. IOSR Journal of Pharmacy and Biological Sciences. Volume 8, Issue 5 (Nov. – Dec.2013), PP 36-40.
  14. Yasmeen Taj, Farhan Essa, Faisal Aziz, Shahana U. Kazmi. Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus. J Infect Dev Ctries 2012; 5(6):403-409.

Corresponding Author

Dr T. Parvathi

Department of Microbiology, Andhra Medical College, Visakhapatnam, India