Title: Influence of Blood Collection Methods on Some Analytes; Potassium and Sodium
Authors: Dr Frederick Igila Allison, Bamigbowu Emmanuel Olugbenga, Dr Shittu Lukman
DOI: https://dx.doi.org/10.18535/jmscr/v8i2.74
Abstract
Background
Haemolysis is a common occurrence in blood sample collection1, the severity of which may compromise the quality of laboratory test results. Many factors may cause haemolysis of blood samples and these include method of blood collection, method of emptying into container, blood anticoagulant ratio, transportation methods and blood sample storage among others. Methods of blood collection are very vital to ensuring quality results from the laboratory, especially for intracellular analytes2. Most times, the method of sample collection is overlooked to the detriment of quality of blood sample and patient care. This is important as haemolysis releases highly concentrated intracellular analytes into the plasma: analytes like potassium, magnesium, phosphate, lactate dehydrogenase among others, 3 have been found to increase in plasma due to haemolysed. Most times in our laboratories and hospitals the visual assessment of the degree of haemolysis is the criteria used to reject haemolysed blood sample. Studies have shown that about 70% of blood sample rejection in our laboratories is due to haemolysis4, and this to a reasonable extent depends on the degree of haemolysis which determines the level of effect of these intracellular substances released into plasma5.
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