Title: Development and Validation of the UV Spectrophotometric Method of Ursodeoxycholic Acid in Methanol
Authors: Asha Devi, Abhishek soni, Dr Amit Chaudhary
DOI: https://dx.doi.org/10.18535/jmscr/v7i10.149
Abstract
New developments have been taking place in the field of Development and validation of analytical method for the estimation of Ursodeoxychlic acid by using Uv spectroscopy. A simple, rapid, accurate and economical UV-spectroscopic method has been developed for the estimation of Ursodeoxycholic. The absorption maxima for Ursodeoxycholic acid is simulated liver fluid was found to be 232 nm. The drug follows linearity in the concentration ranges 1-10 µg/ml at 232nm with a correlation coefficient value of 0.998. The proposed method was applied to liver disease pharmaceutical formulation and % drug estimated was found to be in the range of 99.99% that is good agreement with the lable claim. The accuracy of the method was checked by recovery experiment performed at three levels i.e., 80%, 100%, 120%. The percent recovery was found to be in the range of 90-120%. The low values of % RSD are indicative of accuracy and reproducibility of method. The % RSD ˂ 2 indicates that method is precise.
Keywords: UV- spectroscopy, Ursodeoxycholic acid, Method development, Accuracy, Precision.
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