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Category: Volume 07 Issue 10 October 2019
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Title: Comparison of Antibiotic Profiles of Methicillin Sensitive and Methicillin Resistant Staphylococcus aureus Isolated from Different Specimens of Hospitalized Patients in Dhaka City

Authors: Dr Hafiza Sultana, Dr Humayun Sattar, Dr Shirin Tarafder, Dr Jogendra Nath Sarker, Dr Md. Tofael Hossain Bhuiyan, Dr Md. Mostaqimur Rahman, Dr Md. Abdullah Yusuf

 DOI: https://dx.doi.org/10.18535/jmscr/v7i10.54

Abstract

  

Background: Staphylococcus aureus, resistant to methicillin, has appeared as a pathogen connected with diseases acquired in hospitals and in the community globally.

Objective: The objective of this study was to compare the methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolated from the different samples of hospitalized patients.

Methodology: This cross-sectional study was carried out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University, Dhaka from January 2010 to December 2010 for a period of one (01) year. All patients admitted to hospital were chosen as study population with distinct kinds of diseases. These patients gathered different clinical samples depending on the locations of the diseases. Staphylococcus aureus (S. aureus) was isolated and identified through staining, cultivation and biochemical testing. To detect the methicillin-resistant Staphylococcus aureus, an oxacillin disk diffusion test was performed. PCR wa used to detect the MRSA mecA gene.

Results: Over the period from January 2010 to December 2010, a total of 120 Staphylococcus aureus (S. aureus) isolates were gathered from 266 samples from three hospitals. Patients with age group 61 to 70 years had the largest proportion of incidence of MRSA. The patterns of antimicrobial susceptibility of 19 MRSA strains were reported on disk diffusion. Multi-resistant among them were 14 (73.7 percent) and non-multi-resistant strains were 5 (26.3 percent). Other than the reserve resorts of drugs gentamycin 14(73.7%) were most sensitive followed by cotrimoxazole 11(57.9%), ciprofloxacin 6(31.6%), erythromycin 6(31.6%) and azithromycin 5(26.3%). The site of infection of MRSA was categorized in seven categories depending on the type of specimens. Here skin and soft tissue infection by MRSA strains were predominant and it was 10(14.3%). The incidence of MRSA in three different hospitals was recorded. In BSMMU the incidence was 11 (13.75%), in DMCH 2 (20.0%) and in Popular Diagnostic Center it was 6 (20.0%).

Conclusion: In conclusion, majority MRSA are multiresistant but sensitive towards vancomycin and linezolid.

Keywords: Methicillin sensitive;methicillin resistant; Staphylococcus aureus; hospital-acquired.

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Corresponding Author

Dr Hafiza Sultana